A retrospective study of the culture and sensitivity pattern of urinary tract infection causing organisms at RIMS teaching Hospital, Raichur, India

The objective was to assess the distribution of urinary tract bacterial pathogens and to determine the antibiotic susceptibility profile of the isolates in our setting. Single positive cultures with a colony count of >105 CFU on CLED medium were identified and their antibiotic sensitivity and resistance profile was depicted in the form of an antibiogram.
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A retrospective study of the culture and sensitivity pattern of urinary tract infection causing organisms at RIMS teaching Hospital, Raichur, India Int.J.Curr.Microbiol.App.Sci (2017) 6(10): 1530-1536 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 10 (2017) pp. 1530-1536 Journal homepage: http://www.ijcmas.comOriginal Research Article https://doi.org/10.20546/ijcmas.2017.610.183A Retrospective Study of the Culture and Sensitivity Pattern of Urinary Tract Infection Causing Organisms at RIMS Teaching Hospital, Raichur, India R. Venkatesh Naik*, Basavaraj V. Peerapur and P. Sandhya Department of Microbiology, RIMS, Raichur, Karnataka, India *Corresponding author ABSTRACT A retrospective study was done and culture and sensitivity reports were collected from RIMS, Teaching Hospital, Central Laboratory Microbiology Department, Raichur for a period of Sep 2014 to April 2016 were analysed. A total of 351 urine samples collected during a time period of 20 months from September 2014 to April 2016 and fulfilling the inclusion criteria were included in the study. The objective was to assess the distribution of urinary tract bacterial pathogens and to determine the antibiotic susceptibility profile of the isolates in our setting. Single positive cultures with a colony count of >105 CFU on CLED medium were identified and their antibiotic sensitivity and resistance profile was depictedKeywords in the form of an antibiogram. Out of 351 urine samples 217(61.8%) urine samples wereUrinary tract culture positive with significance. 57.1% of the total isolates were identified as E. coli,infections, E.coli, followed by Pseudomonas spp. 16.1%, Klebsiella pneumonia 9.6%, Proteus spp 4.6%,Antimicrobial Citrobacter spp 4.1%, Staphylococcus aureus 4.6%, Coagulase negative Staphylococcusresistance. 2.3% and Candida spp 1.4%. Among 199 gram negative organism isolated it was observed that 198/199(99.4%) of total tested isolates were sensitive to Imipenem followed byArticle Info Piperacillin/Tazobactam 170/199(85.4%), Cefoperazone/sulbactam 128/199(64.3%),Accepted: Ceftazidime 112/199(56.2%), Augmentin 107/199(53.7%), Amikacin 105/199(52.7%),14 September 2017 Nitrofuration 101/199(50.7%), Cefipime 100/199(50.2%), Ceftriaxone 95/199(47.7%),Available Online: Gentamicin 90/199(45.2%), Nalidixic acid 75/199(37.6%), Levofloxacin 70/199(35.1%),10 October 2017 Ciprofloxacin 48/199(24.1%), Cotrimoxazole 28/199(14.1%). Also out of 18 gram positive organisms isolated 3 were Candida species. Sensitivity profile of gram positive organisms showed Vancomycin was most susceptible 18/18(100%) followed by Augmentin 14/18(77.7%), while Penicillin was least susceptible 4/18(22.2%). This is an alarmingly low sensitivity to Ciprofloxacin, Cotrimoxazole and Penicillin which is routinely used as empirical therapy for uncomplicated community acquired urinary tract infections and other infections. There is need for proper sensitivity testing to guide the management of UTIs with hospital antibiotic policies.IntroductionUrinary tract infections (UTIs) are one of the each year.[4] UTI can be caused by differentmost common bacterial infections in humans microorganisms including bacteria, protozoa,both in the community and hospital setting. [1– parasites, fungi and viruses are the major3] UTI remains second most common causative organisms. Among these bacteriainfectious disease and approximately about account for more than 95% of UTI cases. [5]150 million people were diagnosed with UTI E. coli is the most common isolate 1530 Int.J.Curr.Microbiol.App.Sci (2017) 6(10): 1530-1536responsible for UTI.[6-9] Other important Sample preparation and microscopypathogens are Klebsiella pneumoniae,Pseudomonas aeruginosa and Staphylococcus The co ...