Antimicrobial and antioxidant activities of harmandii Gagnep. collected from Quang Tri province

In order to explore the importance of the Antheroporum genus in pharmaceutical applications, two bioactivities of A. harmandii plant extracts have been evaluated in this study. Antimicrobial and antioxidant activities of A. harmandii extracts will provide evidences for further chemical composition studies to detect new bioactive compounds from this species.
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Antimicrobial and antioxidant activities of harmandii Gagnep. collected from Quang Tri province ACADEMIA JOURNAL OF BIOLOGY 2023, 45(2): 61–69 DOI: 10.15625/2615-9023/18054 ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF Antheroporum harmandii Gagnep. COLLECTED FROM QUANG TRI PROVINCE Nguyen Chi Mai1,2, Ninh Khac Ban1,2, Vu Huong Giang1, Hoang Xuan Diep3, Pham Thi Hoe1, Nguyen Tuong Van4, Tran My Linh1,2,* 1 Institute of Marine Biochemistry, VAST, Vietnam 2 Graduate University of Science and Technology, VAST, Vietnam 3 Department of Agriculture and Rural Development, Dakrong, Quang Tri, Vietnam 4 VNTEST Institute for Quality Testing and Inspection, Vietnam Received 31 January 2023; accepted 30 March 2023ABSTRACT Antheroporum harmandii Gagnep. is a native plant species of Vietnam, belonging to a small genus of the Fabaceae family. In this study, the bioactivities of different extracts from twigs and seeds of A. harmandii plants collected from Dakrong, Quang Tri province were evaluated. Antimicrobial and antioxidant activities of plant extracts had been done using an agar well diffusion method and DPPH assay. The results showed that methanol, dichloromethane and ethyl acetate extracts from twigs and methanol extract from seeds exerted activities to significantly inhibit two out of nine tested microorganisms. Particularly, the dichloromethane extract from the twigs sample exhibited the highest activity to Micrococcus luteus and Staphylococcus aureus strains with inhibition zones of 4.7 ± 0.3 mm and 2.3 ± 0.2 mm, respectively, at concentrations of 50 mg/mL. The lowest MIC values (500 µg/mL) and 50% inhibition concentration values (IC 50) against M. luteus and S. aureus were recorded for the methanol and dichloromethane extracts of twigs. Among tested extracts, the ethyl acetate extract of twigs was the strongest antioxidant, showing radical scavenging potential with IC50 of 117.24 μg/mL. Therefore, A. harmandii species can be suggested as a new source for the isolation of valuable natural compounds. Keywords: Antheroporum harmandii, anti-microbial activity, anti-bacterial activity, natural compounds.Citation: Nguyen Chi Mai, Ninh Khac Ban, Vu Huong Giang, Hoang Xuan Diep, Pham Thi Hoe, Nguyen TuongVan, Tran My Linh, 2023. Antimicrobial and antioxidant activities of Antheroporum harmandii Gagnep. collectedfrom Quang Tri province. Academia Journal of Biology, 45(2): 61–69. https://doi.org/10.15625/2615-9023/18054* Corresponding author email: tmlinh@imbc.vast.vn©2023 Vietnam Academy of Science and Technology (VAST) 61 Nguyen Chi Mai et al.INTRODUCTION Until now, there are only a few biological Plants play an important role in sustaining and/or chemical investigations had been donelife on Earth and provide human with food, for this genus. There were reports of the non-fiber, shelter, medicine, and fuel. Because of protein amino acids arginine andrich in bioactive compounds, plants have homoarginine in seeds of A. pierrei (Evans etphysiological effects such as anticancer, al., 1985). In the selective extract of the leavesantioxidant, anti-inflammatory, and and twigs of A. pierrei, pyranoi soflavonesantimicrobial activities. Nowadays, 25% of including rotenone, 12a-hydroxyrotenone, andmodern medicine come from trees, shrubs or tephrosin have been isolated and exhibitedherbs, which are either directly obtained from strong biological activity to solid tumor cellthe plant extracts or synthesized to mimic lines (Gao et al., 2011). For A. harmandiicompounds derived from plants (Prasad & plants, their seeds were used as a poison forTyagi, 2015). fish and insect by local people. In practice, the seeds were ground and mixed with ash to Antheroporum is a genus of the Fabaceae catch fish or mixed with water and sprayed tofamily, which is ranked the third largest kill lice.family of blossoming plants withapproximately 650 genera and 19,000 In order to explore the importance of thedifferent species, and a big source of bioactive Antheroporum genus in pharmaceuticalcompounds (Tungmunnithum et al., 2021). In ...