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Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways

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An open reading frame (Acc. no. P50740) on theBacillussubtilis chromosome extending from bp 184 997–186 043with similarity to theidi-2 gene ofStreptomycessp. CL190specifying type II isopentenyl diphosphate isomerase wasexpressed in a recombinant Escherichia colistrain. Therecombinant protein with a subunit mass of 39 kDa waspurified to apparent homogeneity by column chromatog-raphy. The protein was shown to catalyse the conversion ofdimethylallyl diphosphate into isopentenyl diphosphate andvice versa at rates of 0.23 and 0.63lmolÆmg)1Æmin)1,respectively, as diagnosed by1H spectroscopy. ...
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Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways

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