Báo cáo khóa học: C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins
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Thioredoxin reductase (TrxR) fromEscherichia coli,themutant proteins E159Y and C138S, and the mutantprotein C138Streated with phenylmercuric acetate werereconstituted with [U-13C17,U-15N4]FAD and analysed, intheir oxidized and reduced states, by13C-,15N- and31P-NMR spectroscopy. The enzymes studied showedvery similar31P-NMR spectra in the oxidized state, con-sisting of two peaks at )9.8 and )11.5 p.p.m. In thereduced state, the two peaks merge into one apparentpeak (at)9.8 p.p.m.)....
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Báo cáo khóa học: C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins
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Báo cáo khóa học: C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins
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