Phân tích tư tưởng của nhân dân qua đoạn thơ: Những người vợ nhớ chồng… Những cuộc đời đã hóa sông núi ta trong Đất nước của Nguyễn Khoa Điềm
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Eleven of the codons specifying the amino acids of the flexible catalyticloop [KRRPRPNVAEVM(197–208)] of Bacillus subtilis phosphoribosyldiphosphate synthase have been changed individually to specify alanine.The resulting variant enzyme forms, as well as the wildtype enzyme, wereproduced in anEscherichia colistrain lacking endogenous phosphoribosyldiphosphate synthase activity and purified to near homogeneity. TheB. subtilisphosphoribosyl diphosphate synthase mutant variants K197Aand R199A were studied in detail....
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Báo cáo khoa học: Catalytic residues Lys197 and Arg199 ofBacillus subtilis phosphoribosyl diphosphate synthase Alanine-scanning mutagenesis of the flexible catalytic loop
