Báo cáo khoa học: Comparison of the substrate specificity of two potyvirus proteases
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The substrate specificity of the nuclear inclusion protein a (NIa) proteolyticenzymes from two potyviruses, the tobacco etch virus (TEV) and tobaccovein mottling virus (TVMV), was compared using oligopeptide substrates.Mutations were introduced into TEV protease in an effort to identify keydeterminants of substrate specificity. The specificity of the mutant enzymeswas assessed by using peptides with complementary substitutions. The crys-tal structure of TEV protease and a homology model of TVMV proteasewere used to interpret the kinetic data....
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Báo cáo khoa học: Comparison of the substrate specificity of two potyvirus proteases
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Báo cáo khoa học: Comparison of the substrate specificity of two potyvirus proteases
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