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Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme

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An efficient bacterial expression system of cyanobacteriumSynechocystissp. PCC 6803 heme oxygenase gene, ho-1,has been constructed, using a synthetic gene. A solubleprotein was expressedat high levels and was highlypurified, for the first time. The protein binds equimolarfree hemin to catabolize the boundhemin to ferric-bili-verdin IXa in the presence of oxygen andreducingequivalents, showing the heme oxygenase activity. Duringthe reaction, verdoheme intermediate is formed with theevolution of carbon monoxide. ...
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Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme

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