Phân tích tư tưởng của nhân dân qua đoạn thơ: Những người vợ nhớ chồng… Những cuộc đời đã hóa sông núi ta trong Đất nước của Nguyễn Khoa Điềm
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The catalytic module ofHypocrea jecorina(previouslyTrichoderma reesei) Cel7B was homologously expressed bytransformation of strain QM9414. Post-translational modi-fications in purified Cel7B preparations were analysed byenzymatic digestions, high performance chromatography,mass spectrometry and site-directed mutagenesis. Of thefive potential sites found in the wild-type enzyme, onlyAsn56 and Asn182 were found to be N-glycosylated.GlcNAc2Man5was identified as the predominant N-glycan,although lesser amounts of GlcNAc2Man7 and glycanscarrying a mannophosphodiester bond were also detected.Repartition of neutral and charged glycan structures overthe two glycosylation sites mainly accounts for the observedmicroheterogeneity of the protein. ...
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Báo cáo khóa học: Heterogeneity of homologously expressed Hypocrea jecorina (Trichoderma reesei ) Cel7B catalytic module
