Báo cáo khóa học: Identification of domains on the extrinsic 23 kDa protein possibly involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II
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To elucidate the domains on the extrinsic 23 kDa proteininvolved inelectrostatic interactionwiththeextrinsic33 kDaprotein in spinach photosystem II, we modified amino orcarboxyl groups ofthe 23 kDa protein to unchargedmethylester groups with N-succinimidyl propionate or glycinemethyl ester in the presence ofa water-soluble carbodi-imide, respectively.TheN-succinimidyl propionate-modified23 kDa protein did not bind to the 33 kDa protein associ-atedwithPSIImembranes, whereas the glycinemethyl ester-modified 23 kDa protein completely bound. ...
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Báo cáo khóa học: Identification of domains on the extrinsic 23 kDa protein possibly involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II
Nội dung trích xuất từ tài liệu:
Báo cáo khóa học: Identification of domains on the extrinsic 23 kDa protein possibly involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II
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