Báo cáo khoa học: Key substrate recognition residues in the active site of a plant cytochrome P450, CYP73A1
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CYP73 enzymes are highly conserved cytochromes P450 inplant species that catalyse the regiospecific 4-hydroxylationofcinnamicacid to formprecursorsofligninandmanyotherphenolic compounds. A CYP73A1 homology model basedon P450 experimentally solved structures was used to iden-tify active site residues likely togovern substrate binding andregio-specific catalysis. The functional significance of theseresidues was assessed using site-directedmutagenesis. Activesite modelling predicted that N302 and I371 form a hydro-gen bond and hydrophobic contacts with the anionic site oraromatic ringofthe substrate....
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Báo cáo khoa học: Key substrate recognition residues in the active site of a plant cytochrome P450, CYP73A1
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Báo cáo khoa học: Key substrate recognition residues in the active site of a plant cytochrome P450, CYP73A1
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