Báo cáo khoa học: Kinetics and thermodynamics of nick sealing by T4 DNA ligase
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T4DNA ligase is an Mg2+-dependent andATP-dependentenzyme that seals DNA nicks in three steps: it covalentlybinds AMP,transadenylates the nick phosphate,and cata-lyses formation of the phosphodiester bond releasing AMP.In this kinetic study,we further detail the reaction mechan-ism,showing that the overall ligation reaction is a super-impositionof twoparallel processes: aprocessiveligation,inwhich the enzyme transadenylates and seals the nickwithoutdissociating fromdsDNA,and anonprocessiveligation,inwhich the enzyme takes part in the abortive adenylationcycle (covalent binding of AMP,transadenylation of thenick,and dissociation)....
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Báo cáo khoa học: Kinetics and thermodynamics of nick sealing by T4 DNA ligase
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Báo cáo khoa học: Kinetics and thermodynamics of nick sealing by T4 DNA ligase
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