Báo cáo khóa học: Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus
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The moderately thermophilic bacteriumAcidithiobacilluscaldus is found in bacterial populations in manybioleaching operations throughout the world. This bac-terium oxidizes elemental sulfur and other reduced inor-ganic sulfur compounds as the sole source of energy. Thepurpose of this study was to purify and characterize thetetrathionate hydrolase of A. caldus. The enzyme waspurified 16.7-fold by one step chromatography using a SPSepharose column. The purified enzyme resolved into asingle band in 10% polyacrylamide gel, both underdenaturing and native conditions. Its homogeneity wasconfirmed by N-terminal amino acid sequencing....
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Báo cáo khóa học: Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus
Nội dung trích xuất từ tài liệu:
Báo cáo khóa học: Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus
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