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Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses

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The information required for successful assembly of anicosahedral virus is encoded in the native conformation ofthe capsidproteinand in its interactionwith the nucleic acid.Here we investigated how the packing and stability of viruscapsids are sensitive to single amino acid substitutions in thecoat protein. Tryptophan fluorescence, bis-8-anilinonaph-thalene-1-sulfonate fluorescence, CD and light scatteringwere employed to measure urea- and pressure-inducedeffects on MS2 bacteriophage and temperature sensitivemutants. ...
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Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses

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