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Báo cáo khóa học: N- and O-linked carbohydrates and glycosylation site occupancy in recombinant human granulocyte-macrophage colony-stimulating factor secreted by a Chinese hamster ovary cell line

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GM-CSF is one of several naturally occurring glycoproteinsthat regulate leukocyte production, migration and function.It has been produced in different cell types, with differentproperties that depend on the production process used. Thepurpose of this work was to characterize the recombinanthuman GM-CSF from an engineered Chinese hamsterovary cell line grown in suspension and as adherent culturefor the identification of the glycosylation sites and the defi-nition of the glycosidic moiety, including the degree of siteoccupancy....
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Báo cáo khóa học: N- and O-linked carbohydrates and glycosylation site occupancy in recombinant human granulocyte-macrophage colony-stimulating factor secreted by a Chinese hamster ovary cell line

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