Báo cáo khoa học: Probing protein–chromophore interactions in Cph1 phytochrome by mutagenesis
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We have investigated mutants of phytochrome Cph1 from the cyanobacter-ium SynechocystisPCC6803 in order to study chromophore–protein inter-actions. Cph1D2, the 514-residue N-terminal sensor module produced as arecombinant His6-tagged apoprotein in Escherichia coli, autoassemblesin vitro to form a holoprotein photochemically indistinguishable from thefull-length product.
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Báo cáo khoa học: Probing protein–chromophore interactions in Cph1 phytochrome by mutagenesis
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Báo cáo khoa học: Probing protein–chromophore interactions in Cph1 phytochrome by mutagenesis
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