Báo cáo khóa học: The structure–function relationship in the clostripain family of peptidases
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In this study we investigate the active-site structure and thecatalytic mechanism of clostripain by using a combinationof three separate techniques: affinity labelling, site-directedmutagenesis and molecular modelling. A benzamidinyl-diazo dichlorotriazine dye (BDD) was shown to act as anefficient active site-directed affinity label for Clostridiumhistolyticumclostripain. The enzyme, upon incubation withBDD in 0.1MHepes/NaOHbuffer pH 7.6, exhibits a time-dependent loss of activity.
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Báo cáo khóa học: The structure–function relationship in the clostripain family of peptidases
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Báo cáo khóa học: The structure–function relationship in the clostripain family of peptidases
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