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Báo cáo khoa học: tmRNA from Thermus thermophilus Interaction with alanyl-tRNA synthetase and elongation factor Tu

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The interaction of aThermus thermophilustmRNA tran-script with alanyl-tRNA synthetase and elongation factorTu has been studied. The synthetic tmRNA was found to bestable up to 70C. The thermal optimum of tmRNAalanylation was determined to be around 50C. At 50C,tmRNA transcript was aminoacylated by alanyl-tRNAsynthetase with 5.9times lower efficiency (kcat/Km)than tRNAAla, primarily because of the difference inturnover numbers (kcat). Studies on EF-Tu protection ofAlatmRNA against alkaline hydrolysis revealed theexistence of at least two different binding sites for EF-Tuon charged tmRNA. ...
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Báo cáo khoa học: tmRNA from Thermus thermophilus Interaction with alanyl-tRNA synthetase and elongation factor Tu

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