Báo cáo khoa học: to 3a-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831 using fluorescence stopped-flow procedures
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The dual nucleotide cofactor-specific enzyme, 3a-hydroxy-steroid dehydrogenase (3a-HSD)from Pseudomonassp.B-0831, is a member of the short-chain dehydrogenase/reductase (SDR)superfamily. Transient-phase kinetic stud-ies using the fluorescence stopped-flow method were con-ducted with 3a-HSD to characterize the nucleotide bindingmechanism. The binding of oxidized nucleotides, NAD+,NADP+and nicotinic acid adenine dinucleotide(NAAD+), agreed well with a one-step mechanism, whilethat of reduced nucleotide, NADH, showed a two-stepmechanism. ...
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Báo cáo khoa học: to 3a-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831 using fluorescence stopped-flow procedures
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Báo cáo khoa học: to 3a-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831 using fluorescence stopped-flow procedures
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