Báo cáo khoa học: Translational incorporation of L-3,4-dihydroxyphenylalanine into proteins
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AnEscherichia coli cell-free transcription⁄translation system was usedto explore the high-level incorporation of l-3,4-dihydroxyphenylalanine(DOPA) into proteins by replacing tyrosine with DOPA in the reactionmixtures. ESI-MS showed specific incorporation of DOPA in place of tyro-sine. More than 90% DOPA incorporation at each tyrosine site wasachieved, allowing the recording of clean15N-HSQC NMR spectra.
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Báo cáo khoa học: Translational incorporation of L-3,4-dihydroxyphenylalanine into proteins
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Báo cáo khoa học: Translational incorporation of L-3,4-dihydroxyphenylalanine into proteins
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