Báo cáo khoa học: Trigger factor interacts with the signal peptide of nascent Tat substrates but does not play a critical role in Tat-mediated export
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Twin-arginine translocation (Tat)-mediated protein trans-port across the bacterial cytoplasmicmembrane occurs onlyafter synthesis and folding of the substrate protein thatcontains a signal peptide with a characteristic twin-argininemotif. This implies that premature contact between the Tatsignal peptide and the Tat translocon in themembranemustbe prevented. We used site-specific photo-crosslinking todemonstrate that the signal peptide of nascent Tat proteinsis in close proximity to the chaperone and peptidyl-prolylisomerase trigger factor (TF). ...
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Báo cáo khoa học: Trigger factor interacts with the signal peptide of nascent Tat substrates but does not play a critical role in Tat-mediated export
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Báo cáo khoa học: Trigger factor interacts with the signal peptide of nascent Tat substrates but does not play a critical role in Tat-mediated export
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