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Báo cáo Y học: Functional assignment of motifs conserved in b1,3-glycosyltransferases A mutagenesis study of murine UDP-galactose: b-N-acetylglucosamine b1,3-galactosyltransferase-I

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Theb1,3-glycosyltransferase enzymes identi®ed to dateshare several conserved regions and conserved cysteine res-idues, all being located in the putative catalytic domain. Toinvestigate the importance of these motifs and cysteines forthe enzymatic activity, 14 mutants of the murine b1,3-galactosyltransferase-I gene were constructed and expressedin Sf9 insect cells. Seven mutations abolished the galacto-syltransferase activity.
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Báo cáo Y học: Functional assignment of motifs conserved in b1,3-glycosyltransferases A mutagenesis study of murine UDP-galactose:b-N-acetylglucosamine b1,3-galactosyltransferase-I

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