Doctoral thesis summary: Study on rice breeding for brown planthopper resistance using molecular markers

Objectives of the dissertation: Applying marker assisted selection method to develop inbred - elite rice varieties which shown a stability of brown planthopper resistance in the Red River Delta areas.
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Doctoral thesis summary: Study on rice breeding for brown planthopper resistance using molecular markers MINISTRY OF EDUCATION MINISTRY OF AGRICULTURE AND TRAINING AND RURAL DEVELOPMENT VIETNAM ACADEMY OF AGRICULTURAL SCIENCES ******* PHUNG TON QUYEN STUDY ON RICE BREEDING FOR BROWN PLANTHOPPER RESISTANCE USING MOLECULAR MARKERS Major Field: Genetics and Plant Breeding Code: 62.62.01.11 DOCTORAL THESIS SUMMARY Hanoi - 2014 The Doctoral thesis was completed in: AGRICULTURAL GENETICS INSTITUTE Scientific Supervisors: 1: Assoc. Prof. Vu Duc Quang 2: Dr. Luu Thi Ngoc Huyen Reviewer 1: Reviewer 2: Reviewer 3: The Doctoral thesis was defended at Institute Committee of PhD Dissertation Examination: ………...………………………………………………………………………………… ………………………………………………………………………….....…………….. Date: The Ph.D thesis may be found at: .....................…………………………………….. THE PUBLISHED ARTICLES RELATED TO THE CURRENT THESIS 1 Phung Ton Quyen, Nguyen Thi Lang, Luu Thi Ngoc Huyen, Vu Duc Quang (2010), “Evaluation of some brown planthopper resistance of rice lines/varieties in Red River and Cuulong deltas”, Journal of Vietnam Science and Agricultural Technology, No: 1 (14)/2010, pp. 8-13 (in Vietnamese) 2 Dinh Van Thanh, Lai Tien Dung, Phan Thi Bich Thu, Luu Thi Ngoc Huyen, Phung Ton Quyen (2011), “Results of evaluating research on brown planthopper resistance Nilaparvata lugens (Homoptera: Delphacidea)”, Final Report of Department of Crops Production, Agricultural Publisher, pp. 256-263 (in Vietnamese). 3. Luu Thi Ngoc Huyen, Phung Ton Quyen, Vu Duc Quang (2013), “Application of marker technology to develop inbred rice with brown planthopper resistance”, Journal of Vietnam Science and Agricultural Technology, No:2 (41)/2013, pp. 20-25 (in Vietnamese). INTRODUCTION 1. Imperativeness of the dissertation Rice (Oryza sativa L) is an important plant in Vietnam and is a major food source for half of worldwide population. One of the feasible strategies is to apply biotechnologies to improve rice yield and generate new rice varieties to ensure the food demand of human. Brown Planthopper (Nilaparvata Lugens Stal) is one of serious pests which causes rice yield significant reduction in the rice producing countries, especially in the tropic areas of rice growing in the world. In Vietnam, approximately 10% to 30% out of total rice production are annually loss due to planthopper. The aim of applying marker assisted selection (MAS) is to use the markers which are linked with the desire QTL/gen to enhance selecting efficiency and shorten the selection time. To develop the rice varieties which are resistant with brown planthopper with MAS, mainly depends on the markers have linkage with the brown planthopper resistant gen is possible work. Hence, we have concentrated on the topic entitled “Study on rice breeding for brown planthopper resistance using molecular markers ” Objectives of the dissertation Applying marker assisted selection method to develop inbred-elite rice varieties which shown a stability of brown planthopper resistance in the Red River Delta areas. 2. Time and areas implementation - From 2007 to 2013 - Agricultural Genetics Institute, Institution of Plant Protection, Center of Technologies Exchange and Extension, Thanh Tri- Ha Noi. - Field works was mainly conducted in Nam Dinh, Thai Binh, Ha Nam, Hanoi, provinces. 3. Inovative and its contributions 1 - This is one of initial researches on improving rice planthopper resistance by applying molecular breeding in Vietnam, it is also one of part involving to Agricultural Biotechnology Project of MARD - Within of the current dissertation, it has been successfully pyramided two genes (planthopper resistant genes) into one rice variety by use of SSR markers which linked to planthopper resistant genes. 4. Significant contributions -Scientific significance: Application molecular markes to generate two rice lines carrying two planthopper resistance genes - Practical significance: The improved line KR8 is a promising line which is possibly released for producing in a large scale. 6. Structure of Dissertation The dissertation was presented in 155 pages which included: Introduction 3 pages, Chapter 1: Background and Scientific Basic (51 pages); Chapter 2: Materials and Methods (23 pages); Chapter 3: Results and Discussion (57 pages), included 01 page presented for conclusions and suggestion, and 01 page listed the published article involving the current dissertation result, and reference list (19 pages). Also, the dissertation included 37 Figures, 37 Tables and 167 liturature cited, in which there are 46 Vietnamese research lituratures and 121 English research articles were used, 03 published articles from the current dissertation and 2 appendixes. Chapter 1 OVERVIEWS 1.1. Brown planthopper and its resistant characterization in rice 1.1.1. Bio-charateristics of BPH The Brown planthopper (BPH) is among the most important pests of rice which has a scientific name as Nilaparvata lugens Stal. 1.1.2. The status of rice losses have caused by BPH in recent years in Vietnam 2 BPH infestation has occurred in recent years, especially in 2000, over 540 thousand hectares of rice were infected by BPH, of which 256 thousand hectares were seriously losses. 1.1.3. Characteristics of BPH resistance in rice: 1.1.3.1. Mechanism of pest resistance ...