Four flavonols from the seed of Hibiscus sabdariffa Linn. growing in Binh Thuan province

The maceration was applied to the seeds of the plant to afford the crude extract which was then fractionated by liquid-liquid extraction to obtain the ethyl acetate extract. The extract was carried out by using normal phase silica gel column chromatography and thin-layer chromatography. Analysis of spectroscopic data including HR-ESI-MS, 1D and 2D-NMR and a comparison of the NMR data with that in the literature led to the structural elucidation of isolated compounds.
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Four flavonols from the seed of Hibiscus sabdariffa Linn. growing in Binh Thuan provinceScience & Technology Development Journal, 22(4):348- 351 Open Access Full Text Article Scientific ReportFour flavonols from the seed of Hibiscus sabdariffa Linn. growingin Binh Thuan ProvincePham Nguyen Kim Tuyen1 , Truong Van Tien2 , Tran The Anh Huy1 , Nguyen Thi Hoa1 , Huynh Bui Linh Chi3 ,Nguyen Thi Hoai Thu4 , Duong Thuc Huy5,* , Nguyen Kim Phi Phung6 ABSTRACT Introduction: Hibiscussabdariffa Linn. (Malvaceae) is a medicinal plant popularly distributed in Asian countries. As of 2019, no chemical investigations from the Vietnamese plant were found. ThisUse your smartphone to scan this paper reported the isolation and elucidation of compounds isolated from the seeds of Hibiscus sab-QR code and download this article dariffa Linn. (Malvaceae) growing in Binh Thuan province. Method: The maceration was applied to the seeds of the plant to afford the crude extract which was then fractionated by liquid-liquid extrac- tion to obtain the ethyl acetate extract. The extract was carried out by using normal phase silica gel column chromatography and thin-layer chromatography. Analysis of spectroscopic data including HR-ESI-MS, 1D and 2D-NMR and a comparison of the NMR data with that in the literature led to the structural elucidation of isolated compounds. Results: Four flavonols comprising quercetin 3- O-α -L-rhamnopyranosyl-(1→6)-β -D-glucopyranoside (1), quercetin 3-O-β -D-glucopyranoside (2),1Faculty of Environmental Science, Sai quercetin (3) and kaempferol (4) were isolated from the ethyl acetate extract. Conclusions: ThisGon University, Ho Chi Minh City is the first time the isolated compounds were found from the seeds of this plant.2 Key words: Hibiscus sabdariffa Linn, Malvaceae, flavonols, quercetin High School Education Office,Department of Education and Trainingof Binh Thuan Province3Department of Nature, Dong Nai INTRODUCTION chromatography and thin-layer chromatography onUniversity, Dong Nai Province the ethyl acetate extract. The chemical structures of4 Hibiscus is one of the largest Malvaceae genera andFaculty of Basic Science, University of isolated compounds were elucidated based on 1D,Medicine and Pharmacy, Ho Chi Minh comprises about 300 species widely distributed in 2D NMR, and MS along with comparison with theCity Central and West Africa, and South East Asia 1,2 . data in the literature. As a result, four compounds5Faculty of Chemistry, Ho Chi Minh City Hibiscus sabdariff a Linn. (Malvaceae) can be usedUniversity of Education, Ho Chi Minh have been afforded, including quercetin 3-O-α -L- as herbal drinks, beverages and flavouring agents.City, Vietnam rhamnopyranosyl-(1→6)-β -D-glucopyranoside (1), Leaves and powdered seeds of H. sabdariffa are used6 Faculty of Chemistry, University of quercetin 3-O-β -D-glucopyranoside (2), quercetin as foods in West Africa 2 . The plant has traditionallyScience, Ho Chi Minh City (3) and kaempferol (4). This is the first time the iso- been used in Mexico for the treatment of hyperten- lated compounds found from the seed of this plant.Correspondence sion, diaphoretic, diuretic and hyperlipidemia 3 . InDuong Thuc Huy, Faculty of Chemistry, Vietnam, this plant is an important medicinal plant 1 MATERIALS AND METHODSHo Chi Minh City University of with folk treatments. Various chemical investiga-Education, Ho Chi Minh City, Vietnam General experimental procedures tions on flowers, calyces, and leaves of H. sabdariffaEmail: huydt@hcmue.edu.vn were reported so far, providing a number of organic The HR-ESI-MS and ESI-MS spectra were carried onHistory compounds such as phenolic acid, pectin, polysac- a Bruker microTOF Q-II and a MSQ plus Mass, re-• Received: 2019-06-25 charides, anthocyanin and flavonoids. Nevertheless, spectively. TLC was carried out on pre-coated silica• Accepted: 2019-11-01• Published: 2019-11-30 the phytochemical data ...