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A sensitive GC¬MS/MS method has been developed and validated for determining ester forms of 3-MCPD and 2¬MCPD in infant formula, using 3¬MCPD¬d5 and 2¬MCPD¬d5 as internal standards, respectively. The esters of MCPDs were extracted from infant formula powder with mixture of ethanol/n-hexane/diethyl ether (1/1/1, v/v/v), then treated with sulfuric acid to release free MCPDs. Free MCPDs and internal standards were derivatized with phenylboronic acid and the corresponding derivatives were used for GC¬MS/MS analysis.
Nội dung trích xuất từ tài liệu:
GC-MS/MS method for simultaneous determination of ester forms of 3-MCPD and 2-MCPD in infant formula
