Influence of plant growth regulators on micropropagation of gwarpatha [Aloe vera (L.) Burm.]

Present investigation was carried out to determine the optimum level of different plant growth regulators for rapid shoot multiplication, rooting and hardening of micropropagated plantlets of Aloe vera. The experiment was laid out using completely randomized design (CRD) with ten replications during 2018-19. Auxiliary shoot explants were inoculated on MS medium supplemented with various concentrations of cytokinin (BAP 0.5 – 6.0 mg/l) alone and BAP (0.5-6.0 mg/l) with auxin (NAA 0.1 – 0.6) in combination for direct shoot bud proliferation. For rooting, auxins (NAA/IBA) were used at the concentrations of 0.5-2.0 mg/l singly and 0.5-2.0 mg/l NAA with IBA at 0.5 and 2.0 mg/l in combinations. Maximum shoot bud induction was obtained when MS medium supplemented with 4.0 mg/l BAP singly and 4.5 mg/l BAP with 0.6 mg/l NAA in combination.
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Influence of plant growth regulators on micropropagation of gwarpatha [Aloe vera (L.) Burm.]