Investigation of the effects of overexpression of Novel_105 miRNA in contrasting potato cultivars during separate and combined drought and heat stresses

Potato (Solanum tuberosum L.) growth, development and production are sensitive to abiotic stress conditions, yet studies about investigation of stress response of potato plants in regulation of gene expression level are limited. The aim of this study was to identify the role of newly identified Novel_105 miRNA in contrasting potato cultivars (tolerant Unica and sensitive Russet Burbank) in response to heat, drought and combined heat and drought stresses using transgenic approach. Transgenic plants of Unica and Russet Burbank were generated with overexpression of pre-miRNA of Novel_105 and both wild type and transgenic T0 lines were subjected to stress. Physiological parameters (gaseous exchange, leaf temperature, chlorophyll content and relative water content) were observed along with biochemical parameters including proline, malondialdehyde and hydrogen peroxide.
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Investigation of the effects of overexpression of Novel_105 miRNA in contrasting potato cultivars during separate and combined drought and heat stresses