Invitro efficacy of bio-agents against gray mold of orange caused by Botrytis cinerea

Three bio-agents i.e. Trichoderma viridae, Trichoderma harzianum and Trichoderma aspergillum were evaluated against Botrytis cinerea under in vitro condition. Culture disc of fungal bio-agents and pathogen were taken from the growing cultures and transferred to PDA contained in 70 mm petridishes on opposite sides approximately at one cm from the wall of the plate. Among these three bio-gents Trichodema harzianum showed best mycelial growth inhibition of Botrytis cinerea.
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Invitro efficacy of bio-agents against gray mold of orange caused by Botrytis cinerea Int.J.Curr.Microbiol.App.Sci (2020) 9(6): 1785-1789 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 6 (2020) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2020.906.222 Invitro Efficacy of Bio-Agents against Gray Mold of Orange caused by Botrytis cinerea Sania Pallabi Reang* Department of Plant Pathology, School of Agricultural, Uttaranchal University, Dehradun-248007, Uttarakhand, India *Corresponding author ABSTRACTKeywords Three bio-agents i.e. Trichoderma viridae, Trichoderma harzianum andBio-agents, Botrytiscinerea, Gray mold, Trichoderma aspergillum were evaluated against Botrytis cinerea under inOrange vitro condition. Culture disc of fungal bio-agents and pathogen were taken from the growing cultures and transferred to PDA contained in 70 mmArticle Info petridishes on opposite sides approximately at one cm from the wall of theAccepted: plate. Among these three bio-gents Trichodema harzianum showed best18 May 2020Available Online: mycelial growth inhibition of Botrytis cinerea.10 June 2020Introduction The origins of most citrus fruits are to be the Southern slope of Himalayan region, theCitrus is the collective term containing a entire north-eastern region of India andnumber of species and varieties of fruits, adjacent China. Oranges have their origin inknown to world over for their characteristic China.flavor and attractive range of colours. Theorange (Citrus sinensis) is the fruit of citrus The major producers of oranges are Mexico,species. Ctrus sinensis is also called as sweet USA, India and Argentina. Oranges areorange which is differentiate it from the bitter extensively grown in Nagpur regions oforange (Citrus aurantium). The juice of Maharashtra, Coorg of Karnataka, Darjeelingorange includes about 12 percent sugars, 0.5- of West Bengal, Sikkim, North-Eastern region1.5 percent titrable acidity and other soluble of India, Himachal Pradesh and Punjab.solids, 1 percent citric acid, about 50 mg Orange is attacked by number of fungal,vitamin C per 100 ml of juice and about 70-90 bacterial, and viral diseases out of which Graypercent water. mold is measured as most important disease 1785 Int.J.Curr.Microbiol.App.Sci (2020) 9(6): 1785-1789for low yields of oranges which is caused by Purification and maintenance of purefungus. Gray mold is also identified as cultureBotrytis rot and bunch rot. It is prevalent allover the world where oranges are grown. The culture of the pathogen was purified byGray mold disease is affected by fungus dividing the single spore and maintained onBotrytis cinerea. PDA slants in refrigerator at 4°C and sub- cultured once in 15 day and used for furtherUnder favorable condition, this fungus attacks studies.all the fleshy tissues having high sugar,however, maximum damage is noticed in Identification of the fungusfruits at the harvest time along with duringtransport and storage. By the attack of The greyish mycelium was observed underBotrytis the mycelium colonizes the fruit the high power (40X) microscope from thecoating and destroys epidermal cells thereby petriplates for confirmation the identity of theagree too much dehydration of water through fungus. Under microscope the branches ofcuticle. conidiophores are shown which have rounded apical cells bearing clusters of conidia. TheThe most prominent symptoms of the disease conidiophores and clusters of conidia lookare found on the fruits. In fruits the first signs like a grape shape cluster and they wereare small, circular, faintly cleared spot. compared with original description of theInfected part of fruits become grey coloured fungus.and show hairy mycelium in grey coloured allabove the surface of fruits. In vitro efficacy of bio control agentsOften the fungus can be seen developing with 20 ml of sterilized PDA medium was platedthe cracks and splits on the fruits. Bunches of in 70 mm petriplates. Culture disc of fungalconidiophores and conidia swell from stomata bio-agents and pathogen were taken from theand peri-stomatal cracks on the skin of the growing cultures and transferred to PDAfruits. contained in 70 mm petridishes on opposite sides approximately at one cm from the wallMaterials and Methods of the plate.Th ...