Nghiên cứu thành phần hóa học của lá cây ráng Tây Sơn Dicranopteris linearis (Burm. F.) Underw.

"Nghiên cứu thành phần hóa học của lá cây ráng Tây Sơn Dicranopteris linearis (Burm. F.) Underw." được thực hiện nhằm khảo sát hóa thực vật của lá cây Dicranopteris linearis mọc ở Lâm Đồng. Bột lá khô Dicranopteris linearis được điều chế thành cao thô. Sau đó, thực hiện chiết lỏng – lỏng cao thô thu được các cao có độ phân cực khác nhau.
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Nghiên cứu thành phần hóa học của lá cây ráng Tây Sơn Dicranopteris linearis (Burm. F.) Underw. TẠP CHÍ KHOA HỌC HO CHI MINH CITY UNIVERSITY OF EDUCATION TRƯỜNG ĐẠI HỌC SƯ PHẠM TP HỒ CHÍ MINH JOURNAL OF SCIENCE Tập 19, Số 10 (2022): 1593-1598 Vol. 19, No. 10 (2022): 1593-1598 ISSN: Website: https://journal.hcmue.edu.vn https://doi.org/10.54607/hcmue.js.19.10.3381(2022) 2734-9918 Research Article * STUDY ON CHEMICAL CONSTITUENTS OF THE LEAVES OF DICRANOPTERIS LINEARIS (BURM. F.) UNDERW. Nguyen Thu Anh1, Nguyen Thi Hoai Thu2, Nguyen Ngoc Khanh Van3, Duong Thuc Huy1* 1 Ho Chi Minh City Universiry of Education, Vietnam 2 University of Medicine and Pharmacy at HCMC, Vietnam 3 Gia Dinh High School, Ho Chi Minh City, Vietnam * Corresponding author: Duong Thuc Huy – Email: huydt@hcmue.edu.vn Received: March 03, 2022; Revised: April 06, 2022; Accepted: October 06, 2022 ABSTRACT Dicranopteris linearis (Burm. F.) Underw. has many popular traditional uses in Asian countries, such as treating ulcers and boils, allergic symptoms, or respiratory troubles. This study investigated the phytochemical of D. linearis grown in Lam Dong Province. The leaf powder of D. linearis was used to prepare a crude extract. This extract was then applied to the liquid-liquid partition to give different polar fractions. The EA fraction was thus applied to silica gel column chromatography to obtain four compounds. Their chemical structures were elucidated by using Nuclear Magnetic Resonance spectroscopy, as well as by the comparison of their NMR data with reported ones. Four compounds consisted of three flavonols, isoquercetin (1), quercetin (2), kaempferol (3), and a sterol, stigmast-5,22-dien-3β-ol-3-O-β-D-glucopyranoside (4). Keywords: Dicranopteris linearis, isoquercetin; kaempferol; quercetin; stigmast-5-en-3β-ol- 3-O-β-D-glucopyranoside 1. Introduction Dicranopteris linearis (Burm. F.) Underw. is a common fern and belongs to the Gleicheniaceae family. They are distributed in Africa and Asia and grow widely in Vietnam, especially in low, hot, and dry mountainous areas (Chi, 2002). The D. linearis extracts showed antidiabetic, anticancer, antibacterial, antioxidant, analgesic, and anti-HIV activities (Li et al., 2006; Li et al., 2008; Chen et al., 2014; Ponnusamy et al., 2015; Zakaria et al., 2016; Duy et al., 2019). In particular, they are widely used as a folk medicine to treat fever (Malaysia), intestinal worms (Indochina), asthma, infertility in women (India), wound (Papua New Guinea) (Kamisan et al., 2014), cough, allergic, and respiratory disorders (Mymensingh) (Sarker et al., 2009). Cite this article as: Nguyen Thu Anh, Nguyen Thi Hoai Thu, Nguyen Ngoc Khanh Van, & Duong Thuc Huy (2022). Study on chemical constituents of the leaves of Dicranopteris linearis (Burm. F.) Underw.. Ho Chi Minh City University of Education Journal of Science, 19(10), 1593-1598. 1593 HCMUE Journal of Science Nguyen Thu Anh et al. This study reported the isolation and structural elucidation of four compounds, including isoquercetin (1), quercetin (2), kaempferol (3), and β-sitosterol 3-O-β-D- glucopyranoside (4) from the leaves of D. linearis collected in Lam Dong Province, Vietnam. OH OH 29 OH OH OH 21 22 4' 19 26 20 1' HO O HO O HO O 7 2 18 27 5 1'' OH OH 4 1 O O OH OH HO OH O 3 OH O 2'' OH OH O OH O HO 7 HO 1' O (1) (2) (3) OH (4) Figure 1. Chemical structures of isolated compounds 1-4 2. Experiment 2.1. General experimental procedures The NMR spectra were recorded on a Bruker Avance 500 spectrometer (500 MHz for 1 H–NMR and 125 MHz for 13C–NMR). n-Hexane, ethyl acetate (EtOAc), methanol (MeOH), and acetone were used to prepare extracts and to elute column chromatography and thin-layer chrom ...