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The effects of four independent variables (pepsin enzyme content, acetic-acid concentration, liquid/solid ratio, and temperature) on the extraction yield of collagen from Pangasius hypophthalmus skin were evaluated. The optimal conditions to obtain the highest yield were determinded as follows: a pepsin enzyme content of 0.25%, a acetic-acid concentration of 0.75 M, a liquid/solid ratio of 80, a temperature of 13.9 oC. The predicted yield was 73% which was in agreement with the actual value (P
Nội dung trích xuất từ tài liệu:
Optimization of conditions for extraction of collagen from the skin of Pangasius hypophthalmus by response surface methodology
