Phytochemical study of Euphorbia cyathophora collected in Dan Phuong, Hanoi and its antidengue activity against DENV1-4 virus serotypes

In this study, the antidengue virus activity of the ethanol extract from the leaves and twigs of E. cyathophora on four virus serotypes DENV1-DENV4 was examined. The result showed that the ethanol extract displayed antidengue activity on DENV1-4 with PRNT50 values of 15.625-62.5 µg/mL. This extract was fractionated to the n-hexane and ethyl acetate fractions.
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Phytochemical study of Euphorbia cyathophora collected in Dan Phuong, Hanoi and its antidengue activity against DENV1-4 virus serotypesCite this paper: Vietnam J. Chem., 2023, 61(3), 371-377 Research articleDOI: 10.1002/vjch.202300123 Phytochemical study of Euphorbia cyathophora collected in Dan Phuong, Hanoi and its antidengue activity against DENV1-4 virus serotypes Nguyen Thi Dung1, Pham Thi Ninh1, Nguyen Thi Luu1, Nguyen The Anh1, Vu Thi Bich Hau2, Nguyen Thi Thu Thuy2, Ho Ngoc Anh3, Phan Dieu Hang4, Tran Thi Phuong Thao1,5* 1 Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay, Hanoi 10000, Viet Nam 2 National Institute of Hygiene and Epidemiology, 1 Yersin Street, Hai Ba Trung, Hanoi 10000, Viet Nam 3 Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay, Hanoi 10000, Viet Nam 4 Hanoi University of Medicine, Nr. 1, Ton That Tung Street, Kim Lien, Dong Da, Hanoi 10000, Viet Nam 5 Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay, Hanoi 10000, Viet Nam Submitted March 30, 2023; Revised May 17, 2023; Accepted May 21, 2023Abstract The ethanol extract from the leaves and twigs of Euphorbia cyathophora showed antiviral activity against four denguevirus serotypes (DENV1-4), with PRNT50 values of 15.625-62.5 µg/mL. Eight compounds including germanicol acetate(1), cymbopogone (2), 1-heptacosanol (3), ethyl α-L-arabinofuranoside (4), quercetin (5), 3-acetoxy-5,7,3’,4’-tetrahydroxyflavone (6), 3-acetoxy-5,7,4’-trihydroxyflavone (7), 4’-acetoxy-3,5,7-trihydroxyflavone (8) were isolatedfrom this extract. Compounds 2-8 were isolated for the first time from Euphorbia cyathophora. Antiviral activityevaluation of compounds 1-3, 5-7 on DENV1-4 serotypes revealed that 7 was the most active compound with PRNT50values of 23.8-95.2 µM. Keywords. Euphorbia cyathophora, antidengue activity, secondary metabolites.1. INTRODUCTION terpenoids.[2,4,6,7] In this study, the antidengue virus activity of the ethanol extract from the leaves andEuphorbia cyathophora (Vietnamese name: Tieu twigs of E. cyathophora on four virus serotypesTrang Nguyen) is a plant belonging to Euphorbiaceae DENV1-DENV4 was examined. The result showedfamily.[1] This plant is widely distributed in tropical that the ethanol extract displayed antidengue activityand subtropical areas, especially in America and on DENV1-4 with PRNT50 values of 15.625-62.5South East Asia. In Vietnam, it is cultivated along the µg/mL. This extract was fractionated to the n-hexanecountry as an ornamental or fence tree. Traditionally, and ethyl acetate fractions. Phytochemicalthe Vietnamese people used E. cyathophora leaves investigation of these fractions led to the isolation offor treatment of boil and scabies diseases, wound eight compounds including germanicol acetate (1),healing, menstrual disorder, detoxification and skin cymbopogone (2), 1-heptacosanol (3), ethyl α-L-bleeding. Its roots and barks are used to cure arabinofuranoside (4), quercetin (5), 3-acetoxy-respiratory disorder and diahera.[1,2] This plant 5,7,3’,4’-tetrahydroxyflavone (6), 3-acetoxy-5,7,4’-possesses diverse biological activities such as trihydroxyflavone (7), 4’-acetoxy-3,5,7-neuroprotection,[2] liver protection,[3] antimicrobial,[4] trihydroxyflavone (8). Except 1, the other sevenanti-inflammation,[4] anti age, anticancer,[2] and anti- compounds have been isolated for the first time fromhyperglycemic activity.[5] Up to now, there are few this plant. Evaluation of antidengue activity ofstudies on the chemical constitution of E. compounds 1-3, 5-7 showed that the flavonoid 7 wascyathophora. Phytochemical investigation of this the most active compound against DENV1-4, withplant showed the presence of flavonoids, phenols and PRNT50 values of 23.8 to 95.2 µM. This work371 Wiley Online Library © 2023 Vietnam Academy of Science and Technology, Hanoi & Wiley-VCH GmbH 25728288, 2023, 3, Downloaded from https://onlinelibrary.wiley.com/doi/10.1002/vjch.20230 ...