Production of interspecific hybrids between pearl millet [Pennisetum glaucum (L.) R. Br.] × Napier Grass [Pennisetum purpureum (K.) Schum] and their characterization

Interspecific hybrids between cultivars of pearl millet [Pennisetum glaucum (L.) R. Br.] with 2n = 2x = 14 and its wild relative Napier Grass [Pennisetum purpureum (K.) Schum] with 2n = 4x = 28, which have multicut behaviour, perennial nature, high biomass and drought tolerance traits for crop improvement, were obtained by cross hybridization. Twenty hybrid progenies were obtained and then examined based on the morphological traits. Nine of them were confirmed to be true interspecific hybrids. The confirmed interspecific hybrids were planted in field and characterized on pollen fertility and cytogenetic basis along with two check varieties. These interspecific hybrids were found to have low pollen viability, probably due to abnormal meiosis.
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Production of interspecific hybrids between pearl millet [Pennisetum glaucum (L.) R. Br.] × Napier Grass [Pennisetum purpureum (K.) Schum] and their characterizationInt.J.Curr.Microbiol.App.Sci (2019) 8(4): 1308-1313 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 8 Number 04 (2019) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2019.804.151 Production of Interspecific Hybrids between Pearl Millet [Pennisetum glaucum (L.) R. Br.] × Napier Grass [Pennisetum purpureum (K.) Schum] and their Characterization Arshpreet Kaur1, Rahul Kapoor1*, Yogesh Vikal2, Anu Kalia3 and Ruchika Bhardwaj1 1 Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141 004, India 2 School of Agriculture Biotechnology, Punjab Agricultural University, Ludhiana 141 004, India 3 Nanotechnology and electron Microscopy Laboratory, Punjab Agricultural University, Ludhiana 141 004, India *Corresponding author ABSTRACTKeywords Interspecific hybrids between cultivars of pearl millet [Pennisetum glaucum (L.)Interspecific R. Br.] with 2n = 2x = 14 and its wild relative Napier Grass [Pennisetumhybrids, purpureum (K.) Schum] with 2n = 4x = 28, which have multicut behaviour,Napier grass, perennial nature, high biomass and drought tolerance traits for crop improvement,Hybridization,pollen viability were obtained by cross hybridization. Twenty hybrid progenies were obtained and then examined based on the morphological traits. Nine of them were confirmed toArticle Info be true interspecific hybrids. The confirmed interspecific hybrids were planted inAccepted: field and characterized on pollen fertility and cytogenetic basis along with two12 March 2019 check varieties. These interspecific hybrids were found to have low pollenAvailable Online: viability, probably due to abnormal meiosis.10 April 2019Introduction promising sources of germplasm that can be grown for biomass production on more thanSustainable strategies are needed to develop 445 million hactares land worldwidebiofuel systems that have high-energy (Campbell et al., 2005).efficiencies, low food security trade-off risksand significant environmental conservation Pearl millet [Pennisetum glaucum (L.) R. Br.]components. Due to their ability to efficiently has been also classified as Pennisetumutilize water and nutrients, perennial grasses typhoideum, Pennisetum americanum orsuch as Pearl millet-Napier grass hybrids Pennisetum spicatum and is locally known as[Pennisetum glaucum (L.) R. Br. × bajra in India. It ranks third after wheatPennisetum purpureum (K.) Schum.] are (Triticum aestivum) and rice (Oryza sativa) 1308 Int.J.Curr.Microbiol.App.Sci (2019) 8(4): 1308-1313and among millets, pearl millet followed (Pereira et al 2001, Mesa Perez et al., 2005,sorghum (Pantulu and Rao 1982). It is the Strezov et al., 2008).basic staple food in the arid and semi-aridregions of India. It is mostly used in poorest Genetically it is tetraploid (2n = 4x = 28,countries and by the poorest people. So, also AABB) species coupled with 4.60 pgknown as the “Poor man’s cereal crop” (Alam genomic DNA content (Martel et al., 1997)et al., 2010). and belongs to the secondary gene pool of this genus. It is an important crop for diaryIt is a kharif crop which grows in hot and dry production in tropic area. In fact it is grownclimates and it can be grown in areas where due to its desirable traits such as droughtthere is deficiency of rainfall. tolerance and wide adaptability to soil conditions (Anderson et al., 2008).Pearl millet is often referred to as the “camelcrop” as it is a tropical plant and also because It has high forage production potentialof its ability to tolerate drought and. In India, coupled with high ...