Purification, refolding, and characterization of recombinant human paraoxonase-1
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A high density lipoprotein (HDL)-linked enzyme with antioxidant and antiatherogenic properties, paraoxonase1(PON1), prevents the formation of atherosclerotic lesions in humans. In the present study, a recombinant hPON1 gene was produced using a small ubiquitin-related modifier (SUMO) fusion protein expression system. To that end, the hPON1 gene was amplified from human liver-ready cDNA, cloned into the expression vector pET SUMO, and expressed in Escherichia coli BL21 (DE3).
Nội dung trích xuất từ tài liệu:
Purification, refolding, and characterization of recombinant human paraoxonase-1
Nội dung trích xuất từ tài liệu:
Purification, refolding, and characterization of recombinant human paraoxonase-1
Tìm kiếm theo từ khóa liên quan:
Journal of Chemistry Recombinant DNA cloning SUMO expression system 6xHis affinity chromatography Small ubiquitin-related modifier (SUMO)Gợi ý tài liệu liên quan:
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