Quantification of lactoferrin in dietary supplements using a Heparin affinity clean-up column by HPLC PDA detector

The use of dietary supplements containing lactoferrin is a growing trend. As a result, there is a need for a simple and reliable analytical method to determine the content of lactoferrin in these supplements to ensure food quality. In this study, an HPLC method was developed and validated for determining lactoferrin in dietary supplements using a HiTrap Heparin clean-up column in combination with HPLC.
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Quantification of lactoferrin in dietary supplements using a Heparin affinity clean-up column by HPLC PDA detector