Study on polysaccharide (1,3)-B-glucan from Ganoderma lucidum

In this paper, a biologically active substance of Lingzhi mushroom was studied. The optima process seperating (1,3)-B-D-glucan from Ganoderma lucidum has been formed and the molecular weight of the preparation is estimated. The structure of (1,3)- -D-glucan unit and its properties are characterized by using HyperChem 7.02 software.
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Study on polysaccharide (1,3)-B-glucan from Ganoderma lucidum Journal of Chemistry, Vol. 43 (2), P. 258 - 262, 2005 study on polysaccharide (1,3)- -glucan from Ganoderma lucidum Received 17th-Sept.-2004 TRAN DINH TOAI1,VU NGOC BAN2, LE CAM TU2, NGUYEN THANH HANH1 1 Institute of Chemistry, Vietnamese Academy of Science and Technology 2 Faculty of Chemistry, Hanoi University of Science SUMMARY In this paper, a biologically active substance of Lingzhi mushroom was studied. The optimal process seperating (1,3)- -D-glucan from Ganoderma lucidum has been formed and the molecular weight of the preparation is estimated. The structure of (1,3)- -D-glucan unit and its properties are characterized by using HyperChem 7.02 software. I - INTRODUCTION Vietnam is rich in highly valuable gene sources in plants and animals which are specific to tropical climate. One of them is Lingzhi mushroom Ganoderma lucidum. Ganoderma lucidum is a precious drug of the Orient medicine, which has been used for thousands of year. Lingzhi mushroom is believed to have extraordinary effects on tumors and cancers. Figure 1: The structure of (1,3)- -D-glucan However, the nature of marvellous drug-lingzhi Modern research has defined that the has never been explained before. Nowadays, the biologically active substance of Lingzhi characterization of the structure of biologically mushroom which can restrain the development active substances has been developed strongly. of cancer cells is (1,3)- -D-glucan. Thus, a The modern methods such as NMR, mass chemotheurapy based on (1,3)- -D-glucan to spectroscopy, HPLC, ..., help us determine treat cancers is a scientific challange now. correctly the structure of biologically active substances of lingzhi mushroom’s constitutions II - MATERIALS AND PRINCIPLES such as alcoloid, terpenoid, steroid, ergosterol, glycol, lectin, several types of polysaccharides 1. Materials (glucose, mantose, galactose) and some metals (Germanium). One of the most important Lingzhi mushrooms were planted on organic compounds of lingzhi mushroom is mushroom farm of Enzyme Department in polysaccharide. Besides, modern medical Institute of Chemistry, Vietnamese Academy of research has defined that the biologically active Science and Technology. substance in Linzghi mushroom which can 2. Principles restrain the development of cancer cells is (1,3)- -D-glucan. (1,3)- -D-glucan is separated with many 258 different solvents: C2H5OH 80%, H2O, collected and dissolved by 15% NaOH solution. (NH4)2C2O4 2%, NaOH 15%, CH3COOH. The solution (IV) was incubated at 20h, 30oC The viscosity method was used in order to and adjusted to pH 9 by using acetic acid 10%. estimate the molecular weight of the obtained Then it was centrifuged to retain the residue and polysaccharide. This is a simply experimental dried. This product was stored and used in method which allows us to value molecular futher experiment. weight in expanse (M~104-106). Mak-Euvin Each step was repeated 3 times and mixed to expression was used to show the dependence of get homologous solution to use in the next step. viscosity on molecular weight: [ ] = KM or The extraction scheme was shown in chart 1. lg[ ] = lgK + lgM [7] where K and are constant which depend on temperature and the nature of solvent. By looking up K, and 10 g of ground fruit-body determining by experiment, the molecular Ethanol 80%, 3h, 100oC weight of the polysaccharide can be valued. 13 C-NMR spectrum is used to define the Sediment I structure of (1,3)- -glucan. 20-30 mg of sample H2O, 5h, 100oC was dissolved in D2O and 1-5% DMSO to the concentration of 2-3% at 80oC. The spectrum Sediment II was defined on Bruker Avent 500 equipment in Institute of Chemistry, Vietnam Academy of (NH4)2C2O4 2%, 3h, 100oC Science and Technology. HyperChem 7.02 software was used to build Sediment III and optimize the geometric structure of (1,3)- - NaOH 15%, 20h, 30oC glucan. Concretely, we use MM+ force field, Polak Ribi ...