Study on Synthesis of chitosan nanoparticles and their application for drug carriers

In this communication, we report the synthesis of chitosan (CS, a biodegradable and biocom- patible natural polymer) [1] nanoparticles as drug carriers by ionic gelation. The method is based on ionic interactions between positively charged groups NH3 + of CS (in dilute CH3COOH solution) and negatively charged of sodium tripolyphos- phate, TPP). This mechanism was confirmed by IR analyses: the presence of the P=O and P-O groups at the frequency of 1180 cm-1 and 1250 cm-1 res- pectively for CS-TPP nanoparticles; and the shifts from 1650 cm-1 and 1595 cm-1 , corresponding to C-O and N-H stretching respec-tively in pure CS, to 1636 cm-1 and 1539 cm-1 for CS-TPP nano- particles, indicated the interaction between CS and TPP [2].
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Study on Synthesis of chitosan nanoparticles and their application for drug carriersShort communication: Study on Synthesis of chitosan nanoparticles and their application for drug carriers Received 2nd-Dec.- 2004 TRAN DAI LAM1, LE THI NGOC LIEN2, VU DINH HOANG1, NGUYEN NGOC THINH, PHAM GIA DIEN2 1 Faculty of Chemical Technology, Hanoi University of Technology 2 Institute of Chemistry, Vietnamese Academy for Science and Technology In this communication, we report the synthesis conventional ones, including improved efficacyof chitosan (CS, a biodegradable and biocom- and selectivity (site specific targeting), reducedpatible natural polymer) [1] nanoparticles as drug side effects and toxicity [3]. The study on processcarriers by ionic gelation. The method is based on of drug release from its carriers will be reported inionic interactions between positively charged following publications.groups NH3+ of CS (in dilute CH3COOH solution)and negatively charged of sodium tripolyphos-phate, TPP). This mechanism was confirmed by IRanalyses: the presence of the P=O and P-O groupsat the frequency of 1180 cm-1 and 1250 cm-1 res-pectively for CS-TPP nanoparticles; and the shiftsfrom 1650 cm-1 and 1595 cm-1, corresponding toC-O and N-H stretching respec-tively in pure CS,to 1636 cm-1 and 1539 cm-1 for CS-TPP nano-particles, indicated the interaction between CS andTPP [2]. To reach the desired particle size, the disso-ciation degrees of CS and TPP must be controlled.These parameters, in their turn, depend on startingconcentrations and especially on pH solutions.Hence, combined pH and UV-vis measurements TEM micrograph of CS-TPP nanoparticleswere carried out, first for TPP and CS solutions (EM-125K, 100 kV, (EM-125K, Voltage: 100separately and then for their mixture, in order to kV, magnification X 100.000)study the nanoparticle formation at different pH. Itwas found that the absorbance variations of these Acknowledgements: This work was supported bysolutions could be correlated to their different a grant from the National Program indegrees of ionization depending on pH values. Nanotechnology (81), for 2005-2006, Ministry ofTEM images showed clearly that the particle size Science and Technology. The authors are gratefulwas in the range of 50 - 70 nm and its distribution to Prof. Acad. Nguyen Van Hieu for his help andwas quite narrow. encouragement. Afterwards, the capacity of CS-TPP nano-particles to associate with bioactive com-pounds REFERENCEShas been demonstrated for BSA (Bovine SerumAlbumin), as a model protein, and for Artesunic 1. M. N. V. Kumar. J. Pharm. Pharmaceut. Sci.,acid, as antimalarial artemisinin derivative. 3(2), 234 - 258 (2000). Being small in size, CS microspheres have 2. G. Socrates. Infrared Characteristic Freque-large surface to volume ratios and can be used for ncies, 2nd-ed., Wiley&Sons (1994).controlled and targeted drug delivery systems, 3. P. Couvreur et al.. Polymeric Nanoparticleswhich offer numerous advantages compared to and Microspheres, CRC Press, 27-93 (1986). i2