Summary of Biology doctoral thesis: Research on oocytes maturation and embryo production from vietnamese Ban native pig by in vitro techniques

Research is conducted on the Ban Pig model with goals: To determine the potential of Ban Pig's oocyte exploitation; to determine cultivate conditions of the oocyte mature in Ban Pig; success in establishing create Pig’s embryo system through in vitro fertilization (IVF) and cloning (C).
Nội dung trích xuất từ tài liệu:
Summary of Biology doctoral thesis: Research on oocytes maturation and embryo production from vietnamese Ban native pig by in vitro techniques MINISTRY OF EDUCATION AND VIETNAM ACADEMY OF TRAINING SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY OF SCIENCE AND TECHNOLOGY ----------------------------- NGUYEN THI NHUNG RESEARCH ON OOCYTES MATURATION AND EMBRYOS PRODUCTION FROM VIETNAMESE BAN NATIVE PIG BY IN VITRO TECHNIQUES Majors: Biotechnology Code: 9 42 02 01 SUMMARY OF PH.D THESIS Hanoi, 2021 Thesis was conducted at: 1. Graduate University of science and technology- Vietnam academy of science and technology 2. Insitute of Biotechnology The first instructor: Ph.D. Bui Xuan Nguyen The second instructot: Ph.D. Nguyen Viet Linh PhD dissertation reviewer 1: PhD dissertation reviewer 2: PhD dissertation reviewer 3: The thesis will be presented in Doctoral Thesis Evaluation Committee meeting at Graduate university of science and technology - Vietnam Academy of Science and Technology at ....hr ......., date ....... month.…… 2021. The thesis can be found at: - Library of Graduate university of science and technology - Vietnam National Library - Institute of Biotechnology AUTHOR’S PUBLICATION RELATED TO THE TOPIC 1. Nguyen Thi Nhung. Nguyen Thi Hong. Nguyen Tien Đat. Nguyen Hoang Thinh. Tamas Somfai. Kazuhiro Kikuchi. Ngo Thi Kim Cuc. Nguyen Thanh Son. Đong Van Quyen. Chu Hoang Ha. Bui Xuan Nguyên. Nguyen Viet Linh. Effects of culture medium on the development of pig embryos obtained by somatics cell nuclear transfer Academia Journal of Biology. 2018. 40(2se):101-105. Doi:10.15625/0866-7160/v40n2se.11610. 2. Nguyen Thi Nhung. Dong Van Quyen. Chu Hoang Ha. Tamas Somfai. Kazuhiro Kikuchi. Ngo Thi Kim Cuc. Nguyen Thi Hong. Bui Xuan Nguyen. Barbara Beck-Woerner. Nguyen Viet Linh. Effect of donor cell type on the development of pig embryos produced by somatic cell nuclear transfer. Journal of Biotechnology 17(2): 1-5. 2019. 3. Nhung Thi Nguyen. Nguyen Xuan Bui. Viet Linh Nguyen. Van Khanh Nguyen. Kazuhiro Kikuchi. Hiep Thi Nguyen. Hong Thi Nguyen. Hoang Thinh Nguyen. Quyen Van Dong. Hoang Ha Chu. Ngo Thi Kim Cuc. Tamas Somfai. Optimization of in vitro embryo production and zygote vitrification for the indigenous Vietnamese Ban pig: the effects of different in vitro oocyte maturation systems. Anim Science Journal. 2020;91:e13412. 4. Nguyen Viet Linh. Thi Hiep Nguyen. Nguyen Thi Nhung. Nguyen Thi Hong. Nguyen Tien Dat. Nguyen Hoang Thinh. Nguyen Khanh Van. Tamas Somfai. Kazuhiro Kikuchi. Optimization of the in vitro fertilization protocol for frozen epididymal sperm with low penetration ability in a native Vietnamese pig. Anim Science Journal. 2018 Aug;89(8):1079-1084.-3. Issue-1. Jan-Feb. 2019 ISSN: 2456-8635. 5. Huynh Thi Huong. Nguyen Thi Nhung. Nguyen Hoang Thinh. Nguyen Viet Linh. The impact of cell lineages gender cultivating in the development of Ban Pig's cloning embryo. The Magazine of Animal Husbandry Association ò Viet Nam. Issue 256 April 2020. 1 INTRODUCTION Development of reproductive biotechnology with the new booming achievements of embryological transfer, in vitro fertilization, cloning, preservation of frozen reproductive and embryonic cells, the interference of reproductive technique and genetic technological therapy on reproductive cells have developed tremendous potential applications in plenty of aspects: improving animal productivity; food security assurance; conservation of biodiversity, the balance of ecosystems and development of modern pharma medical technology. The pigs are one of the most important factors in Biotechnology. They are not only food resources but also are one of the factors in the biological diversity preservation process with giant potential medical applications. Pigs are the big- size animal that has the organ, genome, physiology process, physical and immune system similar to human. So, it is considered that is the most appropriate object for research of transplantation (xenotransplantation). Because of the alive ability of the individual that doesn't carry Porcine Endogenous Retrovirus (PERV), Ban Pig appraised is an important potential object in xenotransplantation technology and the precious genetic genome resources need to be preserved and developed. So we suggest the subject: “ Research on oocytes maturation and embryo production from vietnamese Ban native pig by in vitro techniques”. Goals: Research is conducted on the Ban Pig model with goals: (1). To determine the potential of Ban Pig's oocyte exploitation;(2). To determine cultivate conditions of the oocyte mature in Ban Pig; (3). Success in establishing create Pig’s embryo system through in vitro fertilization (IVF) and cloning (C). Base on researches: (1). Research the structure of ovary and potential of Ban Pig’s oocyte exploitation through the season; (2). Research cultivate oocyte mature by standardized medium method; (3). Research optimization in producing in vitro Ban Pig’s embryos through fertilization medium, condition of fertilization, and embryonic culture; (4). Research produces Ban Pig’s embryos by somatic cell nuclear transfer. The new points in thesis: (1) Thesis provides the new informations about the effects of season, the cultivate medium of oocyte mature, the medium and fertilization regime, cultivate embryos and preservation of frozen in vitro fertilization of embryos, creating cloning embryos by transplantation to establish a successful system of creating Ban Pig's embryos. (2). Being the first research about applying the Piezo method to produce cloning pig’s embryos in Vietnam. ...