Summary of Chemical doctoral thesis: Studying the chemical composition and biological activity of the Sheic species (Callicarpa candicans) and the large-leafed Tuzhou (Callicarpa macrophylla) in Vietnam

Isolate and determine the chemical structure of compounds from C. candicans and C. macrophylla species collected in Vietnam; assessment of toxic activity on some cancer cell lines: liver (Hep-G2), lung (Lu-1) and breast (MCF-7) of isolated clean compounds, researching the chemical composition of C. candicans and C. macrophylla essential oils.
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Summary of Chemical doctoral thesis: Studying the chemical composition and biological activity of the Sheic species (Callicarpa candicans) and the large-leafed Tuzhou (Callicarpa macrophylla) in Vietnam MINISTRY OF EDUCATION VIETNAM ACADEMY AND TRAINING OF SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY SCIENCE AND TECHNOLOGY …………***………… VU THI THU LE RESEARCH ON CHEMICAL CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF CALLICARPA CANDICANS AND CALLICARPA MACROPHYLLA GROWING IN VIETNAM Major: Chemistry of natural compounds Code: 9.44.01.17 SUMMARY OF CHEMICAL DOCTORAL THESIS HA NOI - 2020 This thesis was completed at Graduate University of Science and Technology - Vietnam Academy of Science and Technology. Supervisors: 1. Assoc.Prof. Dr. Pham Thi Hong Minh Institute of Natural Products Chemistry – Vietnam Academy of Science and Technology 2. Prof. Dr. Pham Quoc Long Institute of Natural Products Chemistry – Vietnam Academy of Science and Technology Examiner 1: Assoc.Prof. Dr. Phan Minh Giang University of Science – Vietnam National University Examiner 2: Dr. Đỗ Hữu Nghị Institute of Natural Products Chemistry – Vietnam Academy of Science and Technology The thesis defense was monitored by the Graduate University level Board of Examiners, held at: Graduate University of Science and Technology - 18 Hoang Quoc Viet - Cau Giay - Ha Noi. At ……….. , ….………………….. 2020 The thesis is available in Vietnam National Library and Library of Graduate University of Science and Technology. 1 PREAMBLE 1. The urgency of the thesis Vietnam is located in a tropical climate region with extremely rich natural resources and biodiversity with many valuable medicinal herbs. The increasing use of traditional medicinal herbs or from naturally occurring compounds has taken an important position in medicine. Herbal remedies are the subject for scientists to fully study the nature of the active ingredients in natural plants, the results of the study will contribute to a better explanation of the effects. Treatment of traditional medicinal plants is still often used in folk. In the world, studies of the genus Callicarpa mainly focus on: botanical, pharmacology, plant chemistry and clinical. Pharmacological studies conducted on crude extracts or pure compounds provide the scientific basis for traditional use. Biological activity studies mainly focused on activities: anti- inflammatory, hemostasis, memory loss, oxidation, antibacterial. Studies of chemical composition and biological activity in the world show that diterpenoid and triterpenoid compounds have very good anticancer activity, which is the most abundant chemical component in Callicarpa genus. The research to find out the active ingredients of plants has been receiving the attention of scientists from many countries around the world, including Vietnam. This is also the reason for the topic Studying the chemical composition and biological activity of the Sheic species (Callicarpa candicans) and the large-leafed Tuzhou (Callicarpa macrophylla) in Vietnam. be selected for research. . 2. The research objectives of the thesis Isolate and determine the chemical structure of compounds from C. candicans and C. macrophylla species collected in Vietnam. Assessment of toxic activity on some cancer cell lines: liver (Hep-G2), lung (Lu-1) and breast (MCF-7) of isolated clean compounds. Researching the chemical composition of C. candicans and C. macrophylla essential oils. 3. The main research content of the thesis 1.Determining the chemical structure of compounds isolated from leaves of C. candicans and C. macrophylla in Vietnam 2 2.Determining the essential chemical composition of leaves of C. candicans and C. macrophylla in Vietnam by conventional steam entraining methods and microwave-assisted distillation methods. 3.Evaluation of toxic activity on three cancer cell lines (liver - HepG2, prostate - PC3, lung - A549) and in vitro antimicrobial testing of essential oils obtained from C. candicans leaves and C. macrophylla. 4. Evaluation of toxic activity on three strains of liver cancer (Hep-G2), lung (Lu-1) and breast (MCF-7) in vitro of clean compounds isolated from leaves of C. candicans and C. macrophylla Chapter 1. OVERVIEW The literature review is a collection of national and international research on: 1.1. General characteristics of botany Callicarpa 1.2. Pharmacological effects of the genus Callicarpa 1.3. Chemical composition of plants genus Callicarpa 1.4. Biological activity of genus Callicarpa plants Chapter 2. SUBJECTS AND METHODS OF THE STUDY This section describes in detail the sample handling processes, the methods of generating sediments, chromatography and the isolation of compounds; determine the chemical composition of essential oils and methods of bioactive test. 2.1. Materials and research methods 2.1.1. Plant samples Leaves, stems, branches and fruits (Callicarpa candicans (Burm.f.) Hochr.) And Tu Chau big leaves (Callicarpa macrophylla Vahl) collected in Tam Dao district - Vinh Phuc in October 2015 and Dai Tu district, province Thai Nguyen in October 2017, Dr. Nguyen Quoc Binh (Vietnam Museum of Nature - VAST) identifies the scientific name and denominator model stored at the Vietnam Museum of Nature. 2.1.2. Method of isolation of compounds from tree samples The analysis and separation of plant extracts are made by different chromatographic 3 methods such as thin layer chromatography (TLC), normal column ...