Summary of Chemistry doctoral thesis: Study on chemical constituents and biological activities of several Antidesma species growing in Vietnam

The objectives of the thesis: Study on chemical constituents of three Antidesma species including A. acidum, A. ghaesembilla, and A. hainanensis growing in Vietnam; evaluation of biological activities of isolated metabolites to find potential compounds.
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Summary of Chemistry doctoral thesis: Study on chemical constituents and biological activities of several Antidesma species growing in Vietnam MINISTRY OF EDUCATION VIETNAM ACADEMY AND TRAINING OF SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY SCIENCE AND TECHNOLOGY ----------------------------- LE CANH VIET CUONG STUDY ON CHEMICAL CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF SEVERAL ANTIDESMA SPECIES GROWING IN VIETNAM Major: Organic chemistry Code: 62.44.01.14 SUMMARY OF CHEMISTRY DOCTORAL THESIS Hanoi - 2017 This thesis was completed at: Graduate University Science and Technology - Vietnam Academy of Science and Technology Adviser 1: Assoc. Prof. Dr. Phan Van Kiem Adviser 2: Assoc. Prof. Dr. Le Mai Huong 1st Reviewer: 2nd Reviewer: 3rd Reviewer: The thesis will be defended at Graduate University of Science and Technology - Vietnam Academy of Science and Technology, at hour date month 2017 Thesis can be found in The library of the Graduate University of Science and Technology, Vietnam Academy of Science and Technology. 1 INTRODUCTION 1. The urgency of the thesis The World Health Organization (WHO) had estimated that as much as 80 percent population relies on traditional medicines in deverloping countries, especially medicinal plants. In the process of drugs discovery and development, traditional knowledge and experiential databases were considered to be one of the important factors which could be increased success rate of lead compounds by reducing the consumed time and money spent as well as toxic occurrence. Therefore, investigations on medicinal plants still remain attractive and stimulate attention of scientists worldwide. According to Dictionary of Vietnamese medicinal plants (Vo Van Chi, 2003), in Vietnam, there were 11 species of the Antidesma genus which were used to treat stomachache, syphilis, measles, chickenpox, menstrual diseases, ... Phytochemical investigation of Antidesma genus led to the isolation of numerous alkaloids, terpenoids, steroids, megastigmanes, flavonoids, lignans and other phenolic compounds. In addition, a lot of extractions and isolated compounds exhibited potent anticancer, anti-oxidant, antidiabetic, antimicrobial activity, .... Therefore, thesis title was chosen to be Study on chemical constituents and biological activities of several Antidesma species growing in Vietnam. 2. The objectives of the thesis Study on chemical constituents of three Antidesma species including A. acidum, A. ghaesembilla, and A. hainanensis growing in Vietnam. Evaluation of biological activities of isolated metabolites to find potential compounds. 3. The main contents of the thesis 1. Isolation of compounds from the leaves of A. acidum, A. ghaesembilla, and A. hainanensis; 2. Determination of chemical structures of the isolated compounds; 2 3. Evaluation on the cytotoxic activity of the isolated compounds; 4. Evaluation on the anti-inflammatory activity of isolated compounds. CHAPTER 1: OVERVIEW Overview of internal and international researches related to our study CHAPTER 2: EXPERIMENT AND RESULTS 2.1. Plant materials - The leaves of A. acidum were collectted at Tam Dao, Vinh Phuc, Vietnam in March, 2013. - The leaves of A. ghaesembilla were collectted at Buon Don, Dak Lak, Vietnam in March, 2013. - The leaves of A. hainanensis were collectted at Tam Dao, Vinh Phuc, Vietnam in December, 2014. 2.2. Methods 2.2.1. Methods for isolation of secondary metabolites Chromatographic methods such as thin layer chromatography (TLC), column chromatography (CC). 2.2.2. Methods for determination of chemical struture of compounds Physical parameters and modern spectroscopic methods such as optical rotation ([]D), electrospray ionization mass spectrometry (ESI-MS) and high-resolution ESI-MS (HR-ESI-MS), one/two-dimention nuclear magnetic resonance (NMR) spectra, circular dichroism spectrum (CD). 2.2.3. Methods for evaluation of biological activities - Cytotoxic activity was determined by the MTT assay. - Anti-inflammatory activity of the compounds was assessed on the basis of inhibiting NO production in lipopolysaccharide (LPS) activated BV2 cells. 2.3. Isolation of compounds 2.3.1. Isolation of compounds from A. hainanensis 3 This section presents the process of isolating the compounds from A. hainanensis. Figure 2.4. Isolation of compounds from A. hainanensis 2.3.2. Isolation of compounds from A. acidum This section presents the process of isolating the compounds from A. acidum. Figure 2.5. Isolation of compounds from A. acidum 4 2.3.3. Isolation of compounds from A. ghaesembilla This section presents the process of isolating the compounds from A. ghaesembilla. Figure 2.6. Isolation of compounds from A. ghaesembilla 2.4. Physical properties and spectroscopic data of the isolated compounds 2.4.1. Physical properties and spectroscopic data of the isolated compounds from A. hainanensis This section presents physical properties and spectroscopic data of 18 compounds from A. hainanensis. 2.4.2. Physical properties and spectroscopic data of the isolated compounds from A. acidum This section presents physical properties and spectroscopic data of 12 compounds from A. acidum. 2.4.3. Physical properties and spectroscopic data of the isolated compounds from A. ghaesembilla 5 This section presents physical properties and spectroscopic data of 14 compounds from A. ...