Summary of Chemistry doctoral thesis: Study on chemical constituents and biological activities of Vitex limonifolia Wall. Ex C.B.Clarke and Vitex trifolia L.

Study on chemical constituents of leaves of two Vitex species including Vitex limonifolia Wall. ex C.B.Clarke and Vitex trifolia L. growing in Vietnam; evaluate anti-inflammatory and antiviral activities of isolates to find out bioactive compounds.
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Summary of Chemistry doctoral thesis: Study on chemical constituents and biological activities of Vitex limonifolia Wall. Ex C.B.Clarke and Vitex trifolia L. MINISTRY OF EDUCATION VIETNAM ACADEMY AND TRAINING OF SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY OF SCIENCE AND TECHNOLOGY ----------------------------- NGUYEN THI KIM THOA STUDY ON CHEMICAL CONSTITUENTS AND BIOLOGICAL ACTIVITIES OF Vitex limonifolia WALL. EX C.B.CLARKE AND Vitex trifolia L. Major: Organic chemistry Code: 9.44.01.14 SUMMARY OF CHEMISTRY DOCTORAL THESIS Hanoi - 2019 This thesis was completed at: Graduate University Science and Technology - Vietnam Academy of Science and Technology Advisor 1: Dr. Nguyen Xuan Nhiem Institute of Marine Biochemistry - Vietnam Academy of Science and Technology Advisor 2: Prof. Dr. Ninh Khac Ban Institute of Marine Biochemistry - Vietnam Academy of Science and Technology 1st Reviewer: 2nd Reviewer: 3rd Reviewer: The thesis will be defended at Graduate University of Science and Technology - Vietnam Academy of Science and Technology, at hour date month 2019 The thesis can be found in - The library of the Graduate University of Science and Technology, Vietnam Academy of Science and Technology. - The National Library of Vietnam. 1 INTRODUCTION 1. The urgency of the thesis In the recent decades, the chemical and biological investigation of medicinal plants and animals to find out natural bioactive compounds supplying for pharmaceutical industry is getting more and more attention from scientists worldwide. According to Dictionary of Vietnamese medicinal plants, the species of the Vitex genus were used for treating many diseases, such as: cough, colds, dizziness, headache, asthma, malaria, skin diseases,… [1]. Phytochemical investigation of Vitex genus led to the isolation of numerous terpenoids, flavonoids, ecdysteroids, lignans, and other compounds. Pharmacological studies had shown that the extractions and isolated compounds possess antitumor, anti-inflammatory, antibacterial, antioxidant activities, and so on. However, there are few researches on the chemical components and biological activities of Vitex species growing in Vietnam [2-6]. Therefore, the mission of studying the chemical and bioactive components of Vitex species growing in Vietnam, developing health care production is very important. From above reasons, thesis title was chosen to be “Study on chemical constituents and biological activities of Vitex limonifolia Wall. Ex C.B.Clarke and Vitex trifolia L.” 2. The aim of the thesis Study on chemical constituents of leaves of two Vitex species including Vitex limonifolia Wall. ex C.B.Clarke and Vitex trifolia L. growing in Vietnam. Evaluate anti-inflammatory and antiviral activities of isolates to find out bioactive compounds. 3. The main contents of the thesis 1. Isolate compounds from the leaves of V. limonifolia and V. trifolia; 2 2. Elucidate chemical structures of the isolated compounds; 3. Evaluate the anti-inflammatory activity of the isolated compounds from V. limonifolia; 4. Evaluate the antiviral activity of the isolated compounds from V. limonifolia and V. trifolia. CHAPTER 1: OVERVIEW Overview of national and international researches related to my study of the chemical constituents and biological activities of Vitex genus and about inflammation and antivirus. 1.1. Introduction to Vitex genus The genus Vitex includes approximately 250 known species of trees and shrubs in tropical and sub-tropical regions. In Vietnam, Vitex genus includes 20 species [7]. Since ancient times, civilization used Vitex plants for treating many health problems, such as malaria, herpes, itches, dermatitis or controlling menstruation. The chemical constituents of Vitex include terpenoids, flavonoids, ecdysteroids, lignans, iridoids,…. In Vietnam, there are only 5 published reports about phytoconstituents of some Vitex species, there is no report about biological activities. 1.2. Introduction to inflammation Introduction of inflammation, anti-inflammatory drugs and the role of nitric oxide in inflammatory disease. 1.3. Introduction to antivirus Introduction to antivirus, some types of antiviral drugs are naturally derived. 3 CHAPTER 2: EXPERIMENTAL AND RESULTS 2.1. Plant materials The leaves of Vitex limonifolia Wall. ex C.B.Clarke and Vitex trifolia L. were collected in Bachma National park, Thua Thien Hue, Vietnam in September, 2015. 2.2. Methods 2.2.1. Methods for isolation of secondary metabolites Chromatographic methods such as thin layer chromatography (TLC), column chromatography (CC). 2.2.2. Methods for determination of chemical structure of compounds Physical parameters and modern spectroscopic methods such as optical rotation ([]D), electrospray ionization mass spectrometry (ESI-MS) and high-resolution ESI-MS (HR-ESI-MS), one/two-dimension nuclear magnetic resonance (NMR) spectra, circular dichroism spectrum (CD). 2.2.3. Biological assays - Anti-inflammatory activity of the compounds was assessed on the basis of inhibiting NO production in lipopolysaccharide (LPS) activated BV2 cells. - Antivirus activity was determined by the SRB assay. 2.3. Isolation of compounds This section presents outlines of the general methods to isolate pure substances from the plants samples. 2.3.1. Isolation of compounds from Vitex limonifolia 4 This section presents the process of isolating the compounds f ...