Summary of Chemistry doctoral thesis: Study on isolation and investigation of antibiotic and cytotoxic activity of secondary compounds from three actynomycetes strains Streptomyces G246, G261, G248 collected in some central East Vietnam sea

Summary of Chemistry doctoral thesis: Study on isolation and investigation of antibiotic and cytotoxic activity of secondary compounds from three actynomycetes strains Streptomyces G246, G261, G248 collected in some central East Vietnam sea
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Summary of Chemistry doctoral thesis: Study on isolation and investigation of antibiotic and cytotoxic activity of secondary compounds from three actynomycetes strains Streptomyces G246, G261, G248 collected in some central East Vietnam sea MINISTRY OF EDUCATION AND VIETNAM ACADEMY TRAINING OF SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY OF SCIENCE AND TECHNOLOGY ---------------------------- CAO DUC DANH STUDY ON ISOLATION AND INVESTIGATION OF ANTIBIOTIC AND CYTOTOXIC ACTIVITY OF SECONDARY COMPOUNDS FROM THREE ACTINOMYCETES STRAINS STREPTOMYCES G246, G261, G248 COLLECTED IN SOME CENTRAL EAST VIETNAM SEA Major: Organic Chemistry Code: 9 44 01 14 SUMMARY OF CHEMISTRY DOCTORAL THESIS HA NOI- 2019 This thesis was completed at: Graduate University Science and Technology - Vietnam Academy of Science and Technology Advisor 1: Prof. Dr. Habil. Pham Van Cuong Advisor 2: Dr. Tran Dang Thach 1st Reviewer: 2nd Reviewer: 3rd Reviewer: The thesis will be defended at Graduate University of Science and Technology - Vietnam Academy of Science and Technology, at hour … date … month … 2019 The thesis can be found in - The library of the Graduate University of Science and Technology, Vietnam Academy of Science and Technology. - The National Library of Vietnam. 1 INTRODUCTION 1. The urgency of the thesis The ocean accounts for 70% of the earth's surface, where there is the largest biological diversity on earth. It is home to 34 of the 36 animal and plant sectors on earth with more than 300,000 known species. The marine environment has been known as a rich source of natural compounds, such as a huge pharmaceutical warehouse awaiting exploration and discovery. Characteristics of harsh deep sea habitats are the conditions for forming organic compounds with unique chemical structure characteristics and precious biological activity. The study of secondary active ingredients produced from marine microorganisms in the world has achieved many remarkable achievements. Among them are many secondary compounds with interesting chemical structure and biological activity. At the same time many of these compounds are being tested further for medical applications. Vietnam is located in the Pacific region (with sovereignty with an area of about 1,000,000 km2) with a rich and diverse marine system, with great potential for marine resources. The Government of Vietnam has oriented the development of marine economy, exploitation of natural resources and the study of natural products from the sea. However, the study of secondary compounds from Vietnam's marine microbial source has only just begun, there are very few published studies, although the source of marine diversity of our country is very large. Infectious diseases account for the majority of human and animal diseases. Around the second half of the 19th century, it was discovered that microorganisms are the cause of infectious diseases. Therefore, chemotherapy aims at pathogenic microorganisms that have been developed into the main therapy. In 1928, Alexander Fleming discovered Penicillin - a powerful antibiotic compound and purified by Abraham, Chain, and Florey in a stable form that had a therapeutic effect in 1941. Penicillin antibiotics became famous because It saved many lives in World War II. Since then, antibiotics have become an early miracle pharmaceutical that occupies the world's leading position in the pharmaceutical field, with increasingly new results, with increasing demand and increasing production volume. Currently, there are more antibiotics that are extracted from fungi, bacteria, actinomycetes, which occupy most of them with marine actynomycetes. But more and more microorganisms cause disease resistance to existing antibiotics. Therefore, it is necessary to continue to research, find and discover new antibiotics, active ingredients with tuberculosis and anti-cancer resistance. Therefore, we carried out this thesis with the title: “Study on isolation and investigation of antibiotic and cytotoxic activity of secondary compounds from three actynomycetes strains Streptomyces G246, G261, G248 collected in some central East Vietnam sea” 2 2. The aim of the thesis - Study to isolate and determine the structure of secondary compounds from cultivation of 3 strains of microorganisms isolated from central East Viet Nam sea. - Survey of cytotoxic activity and testing antimicrobial activity of isolated substances as a scientific basis for the research and application of these compounds. 3. The main contents of the thesis - Review of previous studies on secondary compounds as well as biological activity from marine microbial strains. - Finding procedures for handling culture fluid to create extracts. Purify these extracts on column chromatography to obtain fractions. - Refining substances in the segments to obtain compounds. - Determine the chemical structure of isolated compounds. - Testing antimicrobial activity to test isolated substances. - Testing anticancer activity in vitro for some cancer cell lines such as KB, MCF-7, Hep-G2, Lu-1 of compounds isolated. CHAPTER 1. OVERVIEW 1.1. The distribution and diversity of marine microorganisms 1.2. Bacterial radiation and the formation of secondary compounds from actinomycetes 1.3. Common marine bacteriophage families 1.4. Secondary compounds with biological activity from marine bacteriophages 1.4.1. Finding in the world 1.4.1.1. The compounds have antibiotic activity 1.4.1.2. The compounds are active against tuberculosis 1.4.1.3. The compounds with cytotoxic activity 1.4.2. Finding in our cou ...