Summary of Doctoral thesis in Chemistry: Research on chemical compositions and biological activities of (Paramignya trimea (Oliv.) Guill. (Xao tam phan) and (Morinda longissima Y. Z. Ruan

The task of studying their chemical composition and biological activities of Morinda longissima and Paramignya trimera species in our country is important and urgent. From that point, the topic “Research on chemical compositions and biological activities of (Paramignya trimea (Oliv.) Guill. (Xao tam phan) and (Morinda longissima Y. Z. Ruan (Nho dong) '' was chosen.
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Summary of Doctoral thesis in Chemistry: Research on chemical compositions and biological activities of (Paramignya trimea (Oliv.) Guill. (Xao tam phan) and (Morinda longissima Y. Z. Ruan MINISTRY OF EDUCATION VIETNAM ACADEMY OF AND TRAINING SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY OF SCIENCE AND TECHNOLOGY -------------------------------- TRAN THU HUONG RESEARCH ON CHEMICAL COMPOSITIONS AND BIOLOGICAL ACTIVITIES OF PARAMIGNYA TRIMERA (OILV.) GUILL.) AND MORINDA LONGISSIMA Y. Z. RUAN Speciality: Chemistry of Natural Products Code: 9.44.01.17 SUMMARY OF DOCTORAL THESIS IN CHEMISTRY Ha Noi, 2020 1 The thesis was completed at: Graduate University of Science and Technology – Vietnam Academy of Science and Technology Scientific Supervisors 1: Assoc. Prof. Nguyen Manh Cuong Scientific Supervisors 2: Assoc. Prof. Đo Thi Thao Reviewer 1: … Reviewer 2: … Reviewer 3: …. This thesis is going to be defended at the council of doctoral thesis examiners of Graduate University of Science and Technology, Vietnam Academy of Science and Technology 18 Hoang Quoc Viet, Cau Giay, Hanoi, at ..., ...., ...2020. The thesis can be found at: - Library of Graduate University of Science and Technology - National Library 2 INTRODUCTION 1. The necessity of the dissertation subject In Vietnam, seven Paramignya species have been identified, distributed mostly in the south of the country. Paramignya trimera (Oliv.) Guill., local name “Than duoc” or “Xao tam phan”, is an endemic plant in South Vietnam. The stems and roots of the plant have been used in folk medicine to treat liver disease, especially cirrhotic ascites. Morinda longissima Y.Z. Ruan. (Rubiaceae) is a small-to- medium tree, contributed in mountainous area in the North provinces of Vietnam. The stems and roots of medicinal plant Morinda longissima has been used by Thai's ethnic people in Son La and Lai Chau provinces for treatment of liver disorders, collitis and oedema. Therefore, the task of studying their chemical composition and biological activities of Morinda longissima and Paramignya trimera species in our country is important and urgent. From that point, the topic “Research on chemical compositions and biological activities of (Paramignya trimea (Oliv.) Guill. (Xao tam phan) and (Morinda longissima Y. Z. Ruan (Nho dong) '' was chosen. 2. The objectives of the thesis - Study on chemical composition of Paramignya trimera and Morinda longissima species. - Evaluation of hepatoprotective and anti-HBV activities of extracts and pure compounds from stems and roots of Xao tam phan (Paramignya trimera (Oliv.) Guill.) and Nho dong (Morinda longissima Y.Z. Ruan). 3. The main contents of the thesis - Evaluation of hepatoprotective and anti-HBV activity of herbal extracts. - Isolation of compounds from stems and roots of Paramignya trimera (Oliv.) Guill. and Morinda longissima Y.Z. Ruan. - Determination of chemical structures of isolated compounds. 3 - Evaluation of hepatoprotective and anti-HBV activity of isolated compounds. Outline of the thesis: The thesis consists of 139 pages with 45 tables, 21 figures, 4 schemes of isolation and 137 references. The thesis consists of 4 chapters: Introduction (2 pages), Chapter 1: Liturature overview (28 pages); Chapter 2: Materials and Methods (02 pages); Chapter 3: Results (10 pages), Chapter 4: Discussion (83 pages); Conclusion (2 pages); Suggestion (1 page); Publications related to the thesis (1 page); References (15 pages); Appendix (31 pages). CHAPTER 1: LITERATURE OVERVIEW This part reviews the following points: - The chemical composition and biological activities of compounds isolated from Paramignya plants were presented thoroughly. - The chemical compositions and biological activities of compounds isolated from Morinda plants were presented thoroughly. - An overview of biological techniques for screening hepatoprotective and anti-HBV activity was presented in detail. CHAPTER 2: MATERIALS AND METHODS 2.1.Plant Materials The stems and roots of Paramingya trimera and Morinda longissma were collected in Khanh Hoa province and in Son La province, Vietnam, respectively. The plants were identified by the botanist Dr. Nguyen Quoc Binh, Vietnam National Museum of Nature, VAST. Voucher specimens (C-499 and C-547) are deposited in the herbarium of the Institute of Natural Products Chemistry, VAST, Hanoi, Vietnam. 2.2. Method - Phytochemical techniques - Chromatographic TLC analysis on precoated plates of silica gel 60 F254, separation methods including column chromatography on silica gel, C-18 silica gel, Diaion HP-20, Sephadex LH-20 were used to isolate natural compounds from plant extracts. 4 - Structures of isolated compounds were identified by physical, chemical and spectroscopic methods including ESI-MS, HR-ESI- MS, IR, UV, 1D và 2D-NMR. - Evaluation of biological activity The biological activities of the extracts and pure natural compounds were evaluated including: -In vitro anti-HBV activity on HepG2.2.15 cell line based on HBsAg expression levels with 50% inhibitory concentration values (IC50). - In vivo hepatoprotective activities against paracetamol-induced hepatotoxicity in BALB/c mice. CHAPTER 3: EXPERIMENTAL AND RESULTS 3.1. Evaluation of hepatoprotective and anti-HBV activities of herbal extracts 3.2. Isolation of natural compounds from Paramignya trimera From the stems and roots of Paramignya trimera, 10 compounds, including four coumarins: ostruthin (PT-1); nin ...