Summary of doctoral thesis in Material science: Research on the synthesis, properties of GdPO4: Tb3+ and Gd2O3: Eu3+ nanomaterials oriented for biomedical applications

Successful synthesis of GdPO4:Tb3+ nanorods and Gd2O3:Eu3+ nanospheres; coating GdPO4:Tb3+ nanorods and Gd2O3:Eu3+ nanospheres with a silica shell, functionalizing with NH2 functional group, conjugating with anti-venom and anti-CEA antibodies for further biomedical applications.
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Summary of doctoral thesis in Material science: Research on the synthesis, properties of GdPO4:Tb3+ and Gd2O3:Eu3+ nanomaterials oriented for biomedical applications MINISTRY OF EDUCATION VIETNAM ACADEMY OF AND TRAINING SCIENCE AND TECHNOLOGY ----------------------------- GRADUATE UNIVERSITY SCIENCE AND TECHNOLOGY PHAM THI LIEN RESEARCH ON THE SYNTHESIS, PROPERTIES OF GdPO4:Tb3+ AND Gd2O3:Eu3+ NANOMATERIALS ORIENTED FOR BIOMEDICAL APPLICATIONS Major: Optical, optoelectronic and photonic materials Code: 62 44 01 27 SUMMARY OF DOCTORAL THESIS IN MATERIAL SCIENCE Ha Noi - 2020 The thesis has been completed at: Graduate University of Science and Technology - Vietnam Academy of Science and Technology Science supervisors: 1. Dr. Nguyen Thanh Huong 2. Prof. Dr. Le Quoc Minh Reviewer 1: Reviewer 2: Reviewer 3: The thesis was defended at Evaluation Council held at Graduate University of Science and Technology - Vietnam Academy of Science and Technology on , 2020. Thesis can be further referred at: - The Library of Graduate University of Science and Technology. - National Library of Vietnam. 1 INTRODUCTION 1. 1. The necessity of the thesis Luminescent nano-materials containing Rare Earth (RE-NP) have received much attention in recent years. Notably, the materials GdPO4: Tb3+ and Gd2O3:Eu3+ become the critical research tool for the wide- ranging biomedical applications in the diagnosis and treatment of human disease. Magnetic resonance imaging (MRI) examination and especially live-cell fluorescence microscopy are widely utilized in hospitals and clinics. In Vietnam, many research groups have focused on the synthesis of luminescent materials as photonic biomarkers, creating the analytical tool for assessing the quality of biologicals, foods and vaccines. However, most of the biomedical applications are primarily based on a single functional tool, e.g. either luminescent material as a biomarker or magnetic material as a hyperthermia agent for enhancing the contrast of image. Therefore, it is urgent to develop a multifunctional tool, especially simultaneously possessing fluorescence and magnetic properties in a single system to improve the efficiency of diagnostic and treatment processes. According to the latest reports in the field of rare-earth luminescent materials, we decide to chose Terbium (Tb3+) and Europium (Eu3+)-activated Gadolinium (Gd3+) compounds as research objects. To our knowledge, these kinds of materials can meet the requirement of multifunctional tool because Gd3+ ions simultaneously exhibit fluorescence and magnetic properties, thus making them well- suited to serve as nanoprobes in the diagnostic and treatment of human disease. 2 To meet the requirement for diverse applications, the aim of our thesis is to synthesize the luminescent nanomaterials with good repeatability, well-controlled morphology, high solubility in water and highly efficient luminescence. My doctoral thesis is entitled: “Research on the synthesis, properties of GdPO4:Tb3+ and Gd2O3:Eu3+ nanomaterials oriented for biomedical applications”. 2. Research objectives of the thesis - Successful synthesis of GdPO4:Tb3+ nanorods and Gd2O3:Eu3+ nanospheres. - Coating GdPO4:Tb3+ nanorods and Gd2O3:Eu3+ nanospheres with a silica shell, functionalizing with NH2 functional group, conjugating with anti-venom and anti-CEA antibodies for further biomedical applications. - Initial experimental application of the nanocomplexs based GdPO4:Tb3+ nanorods and Gd2O3:Eu3+ nanospheres using immunofluorescence analysis method for labelling cells and detecting the antigen of cobra venom and CEA antigen of colorectal cancer cells. 3. Research contents of the thesis In the following chapter 1, we present a brief overview of rare- earth doped luminescent material; GdPO4:Tb3+/Gd2O3:Eu3+ based luminescent materials and objects for biomedical application. Next, we describe experimental methods and material characterizations used in the thesis. We then present the material characterization results of GdPO4:Tb3+ nanorods and Gd2O3:Eu3+ nanospheres. We also examine and determine the magnetic hyperthermia property of GdPO4 and GdPO4:Tb3+ nanorods. These nanomaterials were then conjugated 3 with anti-venom and anti-CEA antibodies for further biomedical applications. Finally, we demonstrate the great potential of these multifunctional nanocomplexs for detecting cobra venom antigen and the CEA antigen of colorectal cancer cells. We conclude in the last chapter. In this thesis, the luminescent nanomaterials have been synthesized by using hydrothermal process and multistep synthesis approach. Surface morphology and structural characteristics of as- synthesized nanomaterials were examined with field emission scanning electron microscopy (FESEM), high-resolution transmission electron microscopy (HRTEM), energy dispersive X-ray spectroscopy (EDX). The optical properties of as-prepared samples were measured with the spectrometers (iHR550, Acton SP2300i and Fluorolof FL3- 2-2, Horiba (USA)). The magnetic properties were investigated with magnetic field-dependent magnetization measurement using vibrating sample magnetometer (VSM) system. The measurement of magnetic induction heating is performed with the commercial RDO-HFI (IMS). The biological experiments were performed at the Laboratory of Molecular Biology (Vietnam Military Medical Academy). Novel attribution of the thesis: - Successful synthesis of GdPO4:Tb3+ nanorods with a length of 300– 500 nm and width of 10 ...