Summary of the Phd thesis dissertation in Biology: Studying the effect of some factors on the flowering process of Torenia fournieri L. cultured in vitro

The objective of this project is determination the role of a number of factors such as LED light, type and concentration of sugar, plant growth regulators, mineral medium, amino acids and polyamine in the flowering of T. fournieri in vitro.
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Summary of the Phd thesis dissertation in Biology: Studying the effect of some factors on the flowering process of Torenia fournieri L. cultured in vitro MINISTRY OF EDUCATION VIETNAM ACADEMY AND TRAINING SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY OF SCIENCE AND TECHNOLOGY ----------------------------- STUDYING THE EFFECT OF SOME FACTORS ON THE FLOWERING PROCESS OF Torenia fournieri L. CULTURED IN VITRO Area of specialization: Plant physiology Code: 9 42 01 12 THE ABSTRACT OF PHD DISSERTATION IN BIOLOGY HCM CITY – 2020 The dissertation was performed at Institute of Tropical Biology and Tay Nguyen Institute of Scientific Research, Vietnam Academy Science and Technology Supervisor: Prof. PhD. Duong Tan Nhut PhD. Nguyen Huu Ho Reviewer 1: Reviewer 2: Reviewer 3: The dissertation will be defended at the PhD dissertation committee of Graduate University of Science and Technology, Vietnam Academy Science and Technology at on , 2020 The dissertation was deposited at: - Library of Graduate University of Science and Technology - National Library of Vietnam INTRODUCTION T. fournieri is beauty flower, easy care and can be grown for many different purposes such as potted, roadside, indoors, outdoors, grown in cashew, hot, moderate or cold climates. In addition to the ornamental value, T. fournieri is a useful experimental model plant for researchers, especially suitable for research of basic physiological phenomena because of its short life cycle, responding quickly to the change of culture medium. T. fournieri is also used as a source of materials for in vitro studies such as gene transfer, somatic mutation, micropropagation, in vitro flower production... Although there are some researches on flowering of T. fournieri in vitro, studying the role of some factors such as plant growth regulators, carbon sources, mineral nutrient media polyamine, amino acids in the flowering process is necessary to explain more clearly the flowering mechanism of this plant. Plant Cell tissue culture and molecular biology techniques have contributed to the knowledge of flowering processes in plants. So far, besides A. thaliana, a model plant that has been used extensively for flowering research, T. fournieri has also been used as a source of material for in vitro flowering study. In the study of flowering in plants, in vitro culture conditions are ideal for carrying out further studies of this process. Scientists can actively adjust a number of factors such as light intensity, light, temperature, sugar, minerals medium, plant growth regulators in laboratory condition... depending on research purposes. Changes in medium composition, plant growth regulators, or specific changes in culture conditions may accelerate growth rate, shorten the growth period in order to lead early flowering. With this technique, scientists are aiming to further investigate the physiological phenomena 1 of the flowering process. This is an important step on the road to conquer nature. Stemming from the above problems, the topic Studying the effect of some factors on the flowering process of Torenia fournieri L. cultured in vitro was carried out. The objective of the topic The objective of this project is determination the role of a number of factors such as LED light, type and concentration of sugar, plant growth regulators, mineral medium, amino acids and polyamine in the flowering of T. fournieri in vitro. Research content Content 1. Study effect of some factors on the flowering of T. fournieri cultured in vitro. Content 2: Observe changes in morphology, structure of meristem and metabolic activity in the process of flowering of T. fournieri cultured in vitro. CHAPTER 1. LITERATURE REVIEW 1.1. Introduction to the Torenia fournieri (T. fournieri) Classify v Kingdom: Plantae v Phylum: Magnoliophyta v Class: Magnoliopsida v Order: Lamiales v Family: Scrophulariaceae v Genus: Torenia v Species: Torenia fournieri L. The appearance of T. fournieri is described as follows: 2 Stems: green, 15-35 cm in height, often upright. Stems erect, quadrangular, simple or branched above middle, subglabrous Leaves: glossy green, up to 5 cm in length, simple, ovoid (oval), with serrated margin, oppositely arranged on the stem. Flowers: diverse in color and shape. The traditional colors include: purple, blue; the new colors are: white, red, pink, dual color, yellow. Flowers are borne on stalks (pedicels) and may be solitary or may be part of an inflorescence that is classed as a cyme or raceme. T. fournieri is a perennial, dicotyledonous herb, but it is often grown as annual plants. T. fournieri is a diploid plant (n=9). Tetraploid plants (n=18) can be produced by treating the colchicine of plantlet in order to produce commercial varieties with large flowers. However, these tetraploid plants, significantly reduced the viability of pollen, grain formation and uneven chromosome distribution at the anaphase stage when compared to plants bisexual parents. T. fournieri is the favourite flower because of its variety of colors, easy care and is suitable for many purposes such as potted, ornamental, fence or plant to cover the ground. In addition to the decorative value, T. fournieri is also a useful model plant for cytologists to study hybridization because it is easy to perform due to protruding of the embryo sac. T. fournieri is also used to study the position and movement of chromosomes and centromere at an early stage of embryogenesis in hybrid plants. 1.2. The path to flowering in plants The evolutio ...