The effects of PIKfyve inhibitor YM201636 on claudins and malignancy potential of nonsmall cell cancer cells
Số trang: 9
Loại file: pdf
Dung lượng: 8.24 MB
Lượt xem: 6
Lượt tải: 0
Xem trước 2 trang đầu tiên của tài liệu này:
Thông tin tài liệu:
PIKfyve is an evolutionarily conserved lipid and protein kinase enzyme that has pleiotropic cellular functions. The aim of the present study was to investigate the effects of phosphatidylinositol-3-phosphate 5-kinase (PIKfyve) inhibitor, YM201636, on nonsmall cell lung cancer (NSCLC) cells growth, tumorigenicity, and claudin (CLDN) expressions. Three NSCLC cell lines (Calu-1, H1299 and HCC827) were used to compare the effects of YM201636. Cytotoxic effects of YM201636 were analysed using XTT assay. Malignancy potential of cells assesses with wound healing and soft agar colony-forming assays. mRNA and protein expressions of claudins were analysed by qRT-PCR and immunofluorescence staining.
Nội dung trích xuất từ tài liệu:
The effects of PIKfyve inhibitor YM201636 on claudins and malignancy potential of nonsmall cell cancer cells
Nội dung trích xuất từ tài liệu:
The effects of PIKfyve inhibitor YM201636 on claudins and malignancy potential of nonsmall cell cancer cells
Tìm kiếm theo từ khóa liên quan:
Journal of Biology Epidermal growth factor receptor Nonsmall cell lung cancer Lung cancer Phosphoinositide kinase Quantitative reverse-transcription polymerase chain reaction (qRT-PCR)Gợi ý tài liệu liên quan:
-
Lentinan enhances the antitumor effects of Delta-like 1 via neutrophils
11 trang 25 0 0 -
7 trang 21 0 0
-
10 trang 19 0 0
-
15 trang 17 0 0
-
Construction and case study of a novel lung cancer risk index
10 trang 16 0 0 -
11 trang 15 0 0
-
Sex difference in EGFR pathways in mouse kidney-potential impact on the immune system
13 trang 15 0 0 -
13 trang 15 0 0
-
Controversies in the Treatment of Lung Cancer
218 trang 15 0 0 -
15 trang 14 0 0