Bài giảng Kỹ thuật phản ứng sinh học: Chương 3 - Bùi Hồng Quân

Bài giảng Kỹ thuật phản ứng sinh học: Chương 3 Động học phản ứng tạo sinh khối, cung cấp cho người học những kiến thức như: Quá trình tạo sinh khối vi sinh vật; Kỹ thuật nuôi cây theo mẻ; Kỹ thuật nuôi cây theo mẻ có bổ sung cơ chất; Phương trình Monod và Động học tạo sinh khối vi sinh vật. Mời các bạn cùng tham khảo!
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Bài giảng Kỹ thuật phản ứng sinh học: Chương 3 - Bùi Hồng Quân http://buihongquan.comChương 3. Động học phản ứng tạo sinh khối  3.1. Cá c khá i niệ m cơ bả n  3.2. Quá trình tạ o sinh khó i vi sinh vạ t  3.3. Kỹ thuạ t nuoi cá y thêo mể  3.4. Kỹ thuạ t nuoi cá y thêo mể có bỏ sungcơ chá t  3.5. Phương trình Monod và Đọ ng họ c tạ o sinh khó i vi sinh vạ t12/23/2018 Bioreaction engineering. 152 http://buihongquan.com BIOLOGICAL REACTIONS •BIOREACTORSLab Scale Bioreactor Industrial Scale Bioreactor 12/23/2018 Bioreaction engineering. 153 http://buihongquan.com Fermentation Process12/23/2018 Bioreaction engineering. 154 http://buihongquan.comMajor Functions of a Bioreactor1) Provide operation free fromcontamination;2) Maintain a specific temperature;3) Provide adequate mixing and aeration;4) Control the pH of the culture;5) Allow monitoring and/or control ofdissolved oxygen;6) Allow feeding of nutrient solutions andreagents;7) Provide access points for inoculationand sampling;8) Minimize liquid loss from the vessel;9) Facilitate the growth of a wide range oforganisms.Ref;(Allman A.R., 1999: FermentationMicrobiology and Biotechnology)12/23/2018 Bioreaction engineering. 155 http://buihongquan.com Biotechnological Processes Of Growing Microorganisms In A Bioreactor1) Batch culture: microorganisms are inoculated into a fixed volume of medium and as growth takes place nutrients are consumed and products of growth (biomass, metabolites) accumulate.2) Semi-continuous: fed batch-gradual addition of concentratednutrients so that the culture volume and product amount areincreased (e.g. industrial production of baker’s yeast);Perfusion-addition of medium to the culture and withdrawal of anequal volume of used cell-free medium (e.g. animal cellcultivations).3) Continuous: fresh medium is added to the bioreactor at theexponential phase of growth with a corresponding withdrawal ofmedium and cells. Cells will grow at a constant rate under aconstant condition.Bioreaction engineering.12/23/2018 156 http://buihongquan.comBiotechnological processes of growingmicroorganisms in a bioreactor12/23/2018 Bioreaction engineering. 157 http://buihongquan.com A Fermenter / Bioreactor And Its Parts by Genentech, Corporate Communication12/23/2018 Bioreaction engineering. 158 http://buihongquan.com Single System for Anchorage-Dependent and Suspension Cultures New Brunswick Scientific Company12/23/2018 Bioreaction engineering. 159 http://buihongquan.com 1500L-Scale Bioreactors (courtesy of Tanox )12/23/2018 Bioreaction engineering. 160 http://buihongquan.com Batch Culture VS Continuous Culture Continuous systems: limited to single cell protein, ethanol productions, and some forms of waste-water treatment processes. Batch cultivation: the dominant form of industrial usage due to its many advantages. Ref;(Smith J.E, 1998: Biotechnology)12/23/2018 Bioreaction engineering. 161 http://buihongquan.comAdvantages of Batch Culture VS Continuous Culture 1) Products may be required only in a small quantities at any given time. 2) Market needs may be intermittent. 3) Shelf-life of certain products is short. 4) High product concentration is required in broth for optimizing downstream processes. 5) Some metabolic products are produced only during the stationary phase of the growth cycle. 6) Instability of some production strains require their regular renewal. 7) Compared to continuous processes, the technical requirements for batch culture is much easier.12/23/2018 Bioreaction engineering. 162 http://buihongquan.com Fermentation Tech ...