Báo cáo khoa học: 2-Pyrimidinone as a probe for studying the EcoRII DNA methyltransferase–substrate interaction
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EcoRII DNA methyltransferase (M.EcoRII) recognizesthe 5¢…CC*T/AGG…3¢ DNA sequence and catalyzes thetransfer of the methyl group fromS-adenosyl-L-methionineto the C5 position of the inner cytosine residue (C*). Here,we study themechanismof inhibitionofM.EcoRII byDNAcontaining 2-pyrimidinone, a cytosine analogue lacking anNH2group at the C4 position of the pyrimidine ring. Also,DNA containing 2-pyrimidinone was used for probingcontacts ofM.EcoRII with functional groups of pyrimidinebases of the recognition sequence....
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Báo cáo khoa học: 2-Pyrimidinone as a probe for studying the EcoRII DNA methyltransferase–substrate interaction
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Báo cáo khoa học: 2-Pyrimidinone as a probe for studying the EcoRII DNA methyltransferase–substrate interaction
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