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The conformationally coupled mechanism by which ATP is utilized byyeast Hsp90 is now well characterized. In contrast, ATP utilization byhuman Hsp90s is less well studied, and appears to operate differently. Toresolve these conflicting models, we have conducted a side-by-side biochem-ical analysis in a series of mutant yeast and human Hsp90s that have beenboth mechanistically and structurally characterized with regard to the crys-tal structure of the yeast Hsp90 protein.
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: A common conformationally coupled ATPase mechanism for yeast and human cytoplasmic HSP90s
