Báo cáo khoa học: A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding
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Exoribonuclease II (RNase II), encoded by thernb gene, is a ubiquitousenzyme that is responsible for 90% of the hydrolytic activity inEscherichia colicrude extracts. The E. colistrain SK4803, carrying themutant allelernb296, has been widely used in the study of the role ofRNase II. We determined the DNA sequence ofrnb296 and cloned thismutant gene in an expression vector. Only a point mutation in the codingsequence of the gene was detected, which results in the single substitutionof aspartate 209 for asparagine....
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Báo cáo khoa học: A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding
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