Báo cáo khoa học: A steady-state competition model describes the modulating effects of thrombomodulin on thrombin inhibition by plasminogen activator inhibitor-1 in the absence and presence of vitronectin
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Thrombomodulin (TM) slows down the interaction ratebetween thrombin and plasminogen activator inhibitor 1(PAI-1). We now show that the 12-fold reduced inhibitionrate in the presence ofTM does not result from an altereddistribution between PAI-1 cleavage and irreversible com-plex formation. Surface plasmon resonance (SPR) revealedan over 200-fold reduced affinity of TM for thrombin-VR1tPAas compared to thrombin, demonstrating theimportance ofthe VR1 loop in the interaction ofthrombinwithbothTMandPAI-1....
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Báo cáo khoa học: A steady-state competition model describes the modulating effects of thrombomodulin on thrombin inhibition by plasminogen activator inhibitor-1 in the absence and presence of vitronectin
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: A steady-state competition model describes the modulating effects of thrombomodulin on thrombin inhibition by plasminogen activator inhibitor-1 in the absence and presence of vitronectin
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