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Báo cáo khoa học: alanine-extradiol cleavage is followed by intramolecular cyclization in lincomycin biosynthesis

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The LmbB1 protein, participating in the biosynthesis oflincomycin,was heterologously expressed inEscherichia coli,purified in its active form, and characterized as a dimer ofidentical subunits. Methods for purification and analysis ofthe LmbB1 reaction product were developed. Molecularmass and fragmentation pattern of the product revealed bycapillary electrophoresis-mass spectrometry were in agree-ment with its proposed structure, 4-(3-carboxy-3-oxo-propenyl)-2,3-dihydro-1H-pyrrole-2-carboxylic acid. TheLmbB1 is therefore a dioxygenase catalysing the 2,3-extra-diol cleavage of theL-3,4-dihydroxyphenyl alanine aromaticring....
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Báo cáo khoa học: alanine-extradiol cleavage is followed by intramolecular cyclization in lincomycin biosynthesis

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